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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

2170 MilliporeChemiBLOCKER

ChemiBLOCKER MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Key Applications: ELISA, Western Blotting, Immunohistochemistry

Recommended Products

Overview

Replacement Information

Key Spec Table

Key Applications
ELISA, WB, IHC

Description
Catalogue Number	2170
Brand Family	Chemicon®
Trade Name	ChemiBlock Chemicon
Description	ChemiBLOCKER
Overview	The ChemiBLOCKER™ is an unique membrane-blocking agent. As a non-animal protein blocker it offers an alternative to milk-based blocking agents, minimizing the risk of non-specific binding of antibodies to your membrane and lowering your background. ChemiBLOCKER™ may be used at dilutions as high as 20x but for most applications, a 5 to 10 fold dilution is appropriate. ChemiBLOCKER™ is biotin free and will not interfere with ABC detection systems. ChemiBLOCKER™ does contain phosphates and may not be suitable for use with some phospho-specific antibodies. Related Products: • BLOT-QuickBlocker™ A novel blocking protein which produces a semi-clear solution. This allows users the option to examine their blot membranes during the blocking procedure. 200g Catalog Number 2080 • Femto/TBST wash buffer (10X) 250mL Catalog Number 2081 Materials required: • 1X femto/TBST (Catalog Number 2081), 1X TBST, or 1X PBST • primary and secondary antibodies • reagents for immunodetection

References

Product Information
Components	2X ChemiBLOCKER 1 L
HS Code	3824 99 89
Presentation	A 2X solution of non-animal proteins in 2mM TRIS, pH 8.5, 100mM NaCl, 0.001% Tween-20, and 0.08% Kathon added as a preservative.

Applications
Key Applications	ELISA Western Blotting Immunohistochemistry
Application Notes	Western Blot ELISA PROTOCOL: 1. Make up blocking solution by mixing 1 part ChemiBLOCKER™ with 1 part 1X femto-TBST/PBST (TBST or PBST also work) for blocking PVDF membranes. For nitrocellulose membranes, mix 1 part ChemiBLOCKER™ with 3 parts 1X femto-TBST (TBST or PBST also work). Note:ChemiBLOCKER™ can be used at a wide variety of dilutions, some optimization may be necessary. ChemiBLOCKER™ is NOT recommended for phospho-protein studies as it does contain phosphorylated proteins. 2. Incubate the membrane in blocking solution (10-20 mL) for 60 minutes at room temperature with shaking in a heat-sealable plastic bag. 3. Prepare antibody dilution buffer (ADB). For PVDF membranes mix 1 part ChemiBLOCKER™ with 3 parts 1X femto-TBST/PBST (TBST or PBST also work). Incubate the membrane in 5-10 mL primary antibody, diluted appropriately with ADB, for 90 min at room temperature with shaking. 4. Wash the membrane 3 times in 50 mL of 1X femto-TBST (TBST or PBST also work) for 5 min each time at room temperature with shaking. 5. Incubate the membrane in 5-10 mL secondary antibody, diluted appropriately with ADB, for 90 min at room temperature with shaking. 6. Wash the membrane as in step #4, but add one extra wash (4 washes total). 7. Wash the membrane 3 times quickly (30 sec.) in 50 mL pure deionized water each time and then process the blot, using femto-LUCENT™ detection system (or your favorite system).

Applications

Key Applications

ELISA
Western Blotting
Immunohistochemistry

Application Notes

Western Blot

ELISA

PROTOCOL:

1. Make up blocking solution by mixing 1 part ChemiBLOCKER™ with 1 part 1X femto-TBST/PBST (TBST or PBST also work) for blocking PVDF membranes. For nitrocellulose membranes, mix 1 part ChemiBLOCKER™ with 3 parts 1X femto-TBST (TBST or PBST also work). Note:ChemiBLOCKER™ can be used at a wide variety of dilutions, some optimization may be necessary. ChemiBLOCKER™ is NOT recommended for phospho-protein studies as it does contain phosphorylated proteins.

2. Incubate the membrane in blocking solution (10-20 mL) for 60 minutes at room temperature with shaking in a heat-sealable plastic bag.

3. Prepare antibody dilution buffer (ADB). For PVDF membranes mix 1 part ChemiBLOCKER™ with 3 parts 1X femto-TBST/PBST (TBST or PBST also work). Incubate the membrane in 5-10 mL primary antibody, diluted appropriately with ADB, for 90 min at room temperature with shaking.

4. Wash the membrane 3 times in 50 mL of 1X femto-TBST (TBST or PBST also work) for 5 min each time at room temperature with shaking.

5. Incubate the membrane in 5-10 mL secondary antibody, diluted appropriately with ADB, for 90 min at room temperature with shaking.

6. Wash the membrane as in step #4, but add one extra wash (4 washes total).

7. Wash the membrane 3 times quickly (30 sec.) in 50 mL pure deionized water each time and then process the blot, using femto-LUCENT™ detection system (or your favorite system).

Biological Information

Physicochemical Information

Dimensions
Volume	1 L

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store kit components at 2-8°C for up to 1 year from date of purchase. Warm ChemiBLOCKER™ to room temperature and mix thoroughly before dispensing. To avoid possible microbial contamination, dispense all solutions aseptically. Over time, phenolic compounds may precipitate out of solution resulting in the formation of a small amount of dark brown to black particulate material at the bottom of the container. This material will not affect the performance of the blocking compound.

Storage and Shipping Information

Storage Conditions

Store kit components at 2-8°C for up to 1 year from date of purchase. Warm ChemiBLOCKER™ to room temperature and mix thoroughly before dispensing. To avoid possible microbial contamination, dispense all solutions aseptically. Over time, phenolic compounds may precipitate out of solution resulting in the formation of a small amount of dark brown to black particulate material at the bottom of the container. This material will not affect the performance of the blocking compound.

Packaging Information
Material Size	2 X 500 mL
Material Package	sufficient for 100 mini gel blots

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
2170	04053252332289

Documentation

ChemiBLOCKER SDS

Title
Safety Data Sheet (SDS)

ChemiBLOCKER Certificates of Analysis

Title	Lot Number
ChemiBLOCKER TM - 2055959	2055959
ChemiBLOCKERTM	3046799
ChemiBLOCKERTM	2470376
ChemiBLOCKERTM - 2902586	2902586
ChemiBLOCKERTM - 3189229	3189229
ChemiBLOCKERTM - 3209189	3209189
ChemiBLOCKERTM - 3245177	3245177
ChemiBLOCKERTM - 3387662	3387662
ChemiBLOCKERTM - 3403843	3403843
ChemiBLOCKERTM - 3515622	3515622

References

Reference overview	Application	Pub Med ID
Suppression of non-specific binding in serological Luminex assays Tim Waterboer *, Peter Sehr 1, Michael Pawlita, Infection and Cancer Program, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 242, 69120 Heidelberg, Germany Journal of Immunological Methods 309 (2006) 200– 204 2006	Multiplexing
Multiplex human papillomavirus serology based on in situ-purified glutathione s-transferase fusion proteins Waterboer, Tim, et al Clin Chem, 51:1845-53 (2005) 2005		16099939
Widespread thalamic terminations of fibers arising in the superficial medullary dorsal horn of monkeys and their relation to calbindin immunoreactivity. Graziano, Alessandro and Jones, Edward G J. Neurosci., 24: 248-56 (2004) 2004 Show Abstract The relay of pain fibers from the spinal and medullary dorsal horn in the thalamus has become a controversial issue. This study analyzed the relationship of fibers arising in lamina I to nuclei in and around the caudal pole of the ventral posterior nuclear complex and especially to a zone of calbindin-dense immunoreactivity (VMpo) identified by some authors as the sole thalamic relay for these fibers. We show that the densest zone of calbindin immunoreactivity is part of a more extensive, calbindin-immunoreactive region that lies well within the medial tip of the ventral posterior medial nucleus (VPM), as delineated by other staining methods, and prove that the use of different anti-calbindin antibodies cannot account for differences in interpretations of the organization of the posterior thalamic region. By combining immunocytochemical staining with anterograde tracing from injections involving lamina I, we demonstrate widespread fiber terminations that are not restricted to the calbindin-rich medial tip of VPM and show that the lamina I arising fibers are not themselves calbindin immunoreactive. This study disproves the existence of VMpo as an independent thalamic pain nucleus or as a specific relay in the ascending pain system.		14715957

Data Sheet

Title
ChemiBLOCKER™ A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS

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