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14-347M CHK2 Protein, active, 250 µg

Active, recombinant human CHK2, residues 5-543, containing N-terminal GST & C-terminal His-6 tags, for use in Kinase Assays.

CHK2 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-347M
250 µg  Also available in 10 μg size (cat#14-347) and in bulk (cat# 14-347-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-347M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCHK2 Protein, active, 250 µg
OverviewRecombinant human CHK2, residues 5-543, containing N-terminal GST and Cterminal His6 tags
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant human CHK2, residues 5-543, containing N-terminal GST & C-terminal His-6 tags, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in E. coli
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • CHEK2
  • HuCds1
  • CHK2
  • LFS2
  • RAD53
  • bA444G7
  • CDS1
  • OTTHUMP00000028871
  • PP1425
  • Cds1
Protein TargetCHK2
Purification Methodglutathione-agarose and Ni2+/NTA-agarose
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
COFACTOR: Magnesium.
SIZE: 543 amino acids; 60915 Da
SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus.
TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain.
Molecular Weight89.6kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of the CHKtide substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-347) and in bulk (cat# 14-347-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-347M 04053252279713

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Alternative Packsize

Catalogue Number Description
14-347 CHK2 Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases