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SCT214 BioTracker Orange-NHS Live Cell pH dye

Live Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells.

SCT214
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT214
DescriptionBioTracker Orange-NHS Live Cell pH dye
OverviewBioTracker Orange-NHS Live Cell pH dye is a chemical probe which reversibly fluoresces upon acidic pH. The probe is modified with N-hydroxysuccimide ester (NHS) which forms a covalent bond with primary amines just by mixing. It can be used for labeling proteins, antibodies and other molecules. For example, it can be applied for visualizing endocytosis of target protein on cell membrane by labeling antibodies that binds to the target protein. This probe binds to primary amines just by mixing. Thus it can be used to label antibodies and other proteins which have lysine residues. Its fluorescence intensity increases ~20-fold at an acidic pH.

Spectral Properties
Absorbance (max): 544 nm
Emission (max): 565 nm (Orange)

References
Asanuma D et al. Acidic-pH-Activable Fluorescence Probes for Visualizing Exocytosis Dynamics. Angew. Chem Int Ed Engl. 2014 Jun 10; 53(24): 6085-9.
Alternate Names
  • AcidiFluor™ ORANGE NHS
References
Product Information
PresentationLyophilized.
Quality LevelMQ200
Applications
ApplicationLive Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells.
Key Applications
  • Cell Based Assays
  • Antibody Labeling
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 80% (LC)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker Orange-NHS Live Cell pH Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT214 04061841697640