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531370 BMP-2 Signaling Enhancer, A01 - Calbiochem

Enhances BMP-2 associated signaling by inhibiting Smurf1-mediated Smad1/5 ubiquitination and thereby prolonging the half-life of Smad1 and 5. in 48 h).

BMP-2 Signaling Enhancer, A01 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (4-((4-Chloro-3-(trifluoromethyl)phenyl)sulfonyl)-1-piperazinyl)(4-(5-methyl-1H-pyrazol-1-yl)phenyl)methanone, Smurf1 Inhibitor, A01

Primary Target: Smurf1
531370
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₂H₂₀ClF₃N₄O₃S

Products

Catalogue NumberPackaging Qty/Pack
5313700001 Glass bottle 10 mg
Description
OverviewA cell-permeable phenylsulfonyl-piperazinyl compound that enhances BMP-2 associated signaling by selectively inhibiting Smurf1-mediated Smad1/5 ubiquitination and thereby prolonging the half life of Smad1 and 5. Directly and specifically blocks WW domains mediated Smurf1-Smad1/5 complex formation (Kd = 3.664 nM), but has no effect on Smurf2-Smad2 and Smurf2-Smad3 interaction. Increases Smad1/5 levels in cells pre-transfected wild type Smurf1, but not the C699A mutant Smurf1 lacking ubiquitin ligase activity. Does not affect the mRNA levels of Smad1 and Smad5. Also, has no effect on the level of Smad2/3, Smad4, ING2 and RhoA. Shown to potentiate BMP-2 induced osteoblastic differentiation of C2C12 cells (~ 2 µM in 48 h).

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number531370
Brand Family Calbiochem®
Synonyms(4-((4-Chloro-3-(trifluoromethyl)phenyl)sulfonyl)-1-piperazinyl)(4-(5-methyl-1H-pyrazol-1-yl)phenyl)methanone, Smurf1 Inhibitor, A01
References
ReferencesCao, Y., et al. 2014. Sci. Rep. 4, 4965.
Product Information
FormYellow powder
Hill FormulaC₂₂H₂₀ClF₃N₄O₃S
Chemical formulaC₂₂H₂₀ClF₃N₄O₃S
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary Target Smurf1
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
5313700001 04055977260281

Documentation

BMP-2 Signaling Enhancer, A01 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Cao, Y., et al. 2014. Sci. Rep. 4, 4965.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-July-2014 JSW
Synonyms(4-((4-Chloro-3-(trifluoromethyl)phenyl)sulfonyl)-1-piperazinyl)(4-(5-methyl-1H-pyrazol-1-yl)phenyl)methanone, Smurf1 Inhibitor, A01
DescriptionA cell-permeable phenylsulfonyl-piperazinyl compound that enhances BMP-2 associated signaling by selectively inhibiting Smurf1-mediated Smad1/5 ubiquitination and thereby prolonging the half life of Smad1 and 5. Directly and specifically blocks WW domains mediated Smurf1-Smad1/5 complex formation (Kd = 3.664 nM), but has no effect on Smurf2-Smad2 and Smurf2-Smad3 interaction. Increases Smad1/5 levels in cells pre-transfected wild type Smurf1, but not the C699A mutant Smurf1 lacking ubiquitin ligase activity. Does not affect the mRNA levels of Smad1 and Smad5. Also, has no effect on the level of Smad2/3, Smad4, ING2 and RhoA. Shown to potentiate BMP-2 induced osteoblastic differentiation of C2C12 cells (~ 2 µM in 48 h).
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₂H₂₀ClF₃N₄O₃S
Purity≥97% by HPLC
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesCao, Y., et al. 2014. Sci. Rep. 4, 4965.