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GF136 BAFF Protein, Recombinant human

The Human BAFF protein (B-cell activating factor belonging to the TNF family) is a novel ligand in the TNF family.

BAFF Protein, Recombinant human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture, Stem Cell Culture UniProt Number: Q9Y275 Entrez Gene Number: NM_006573.3
GF136
20 µg  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULT, CultHuman E.coliGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of BAFF.
Description
Catalogue NumberGF136
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionBAFF Protein, Recombinant human
OverviewHuman BAFF (B-cell activating factor belonging to the TNF family) is a novel ligand in the TNF family. BAFF plays an important role as co-stimulator of B-cell proliferation and function. Recombinant human BAFF is a soluble 17.0 kDa protein containing 153 amino acid residues, comprising the TNF-like extracellular domain of BAFF.
Alternate Names
  • B-cell Activation Factor
  • TALL-1
  • THANK
  • BLyS
Product Information
PresentationLyophilized from sterile filtered 0.5X PBS, pH 7.5, and 0.5 mM DTT, containing no additives.
Quality LevelMQ100
Applications
ApplicationThe Human BAFF protein (B-cell activating factor belonging to the TNF family) is a novel ligand in the TNF family.
Key Applications
  • Cell Culture
  • Stem Cell Culture
Biological Information
PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of BAFF.
SourceE.coli
Specific ActivityHuman BAFF is biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on human RPMI 8226 cells is 5-10 ng/mL.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.
Gene Symbol
  • TNFSF13B
  • BLyS
  • TNFSF20
  • CD257
  • BAFF
  • TALL1
  • DTL
  • TALL-1
  • ZTNF4
  • THANK
  • BLYS
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9Y275 # Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
SIZE: 285 amino acids; 31223 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted.
TISSUE SPECIFICITY: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
PTM: The soluble form derives from the membrane form by proteolytic processing. & N-glycosylated.
SIMILARITY: SwissProt: Q9Y275 ## Belongs to the tumor necrosis factor family.
Stem Cell Type
  • Human Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight17.0 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. Reconstituted material can be stored at 2°-8°C for one week, but undiluted aliquots at -20°C are recommended. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size20 µg
Global Trade Item Number
Catalogue Number GTIN
GF136 04053252625329