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ABE2871 Anti-phospho-Smad1 (Ser463 Antibody, Ser465)

Anti-phospho-Smad1 (Ser463, Ser465), Cat. No. ABE2871, is a highly specific rabbit polyclonal antibody that targets Mothers against decapentaplegic homolog 1 (Smad1) and has been tested in Immunoprecipitation and Western Blotting.

Anti-phospho-Smad1 (Ser463 Antibody, Ser465) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SMAD1, BSP1, MADH1, MADR1 UniProt Number: Q15797
ABE2871
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABE2871
DescriptionAnti-phospho-Smad1 (Ser463 Antibody, Ser465)
Alternate Names
  • Mothers against decapentaplegic homolog 1
  • MAD homolog 1
  • Mothers against DPP homolog 1
  • JV4-1
  • Mad-related protein 1
  • SMAD family member 1
  • hSMAD1
  • Transforming growth factor-beta-signaling protein 1
  • BSP-1
Background InformationMothers against decapentaplegic homolog 1 (UniProt: Q15797; also known as MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, SMAD1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1) is encoded by the SMAD1 (also known as BSP1, MADH1, MADR1) gene (Gene ID: 4086) in human. Smad1 is a transcriptional modulator that is activated by BMP (bone morphogenetic protein) type 1 receptor kinase. Smad1 is a receptor-regulated Smad (R-Smad) that is found in a complex with Smad4 and YY1. Upon phosphorylation at the C-terminal by BMP type 1 receptor kinase it forms a trimer with another Smad1 and Smd4. The C-terminal phosphoserines of Smad1 are recognized by the Mad Homology 2 (MH2) domain of Smad4. The Smd1/Smad4 complex then translocates to the nucleus where Smad proteins bind to their cognate DNA binding sites with low affinity. This binding is further enhanced in the presence of transcriptional co-activators. Smad1 is ubiquitinated by Smad-specific E3 ubiquitin ligase SMURF1, leading to its degradation via proteasome Smad1 variants are associated with susceptibility to pulmonary hypertension that is characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles.
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.01% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Smad1 (Ser463, Ser465), Cat. No. ABE2871, is a highly specific rabbit polyclonal antibody that targets Mothers against decapentaplegic homolog 1 (Smad1) and has been tested in Immunoprecipitation and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot detected phospho-Smad1 (Ser463, Ser465) in constitutively active ALK6 version in 35S metabolic labeled COS cells (Persson, U., et. al. (1998). FEBS Lett. 434(1-2):83-7).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 8 amino acids surrounding pSer463/pSer465 from the C-terminus region of human Smad1.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Smad1 phosphorylated on Ser463 and Ser465. it targets an epitope within 8 amino acids surrounding Ser463 and Ser465.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMAD1
  • BSP1
  • MADH1
  • MADR1
Modifications
  • Phosphorylation
  • Phosphorylation
Purification MethodUnpurified
UniProt Number
Molecular Weight~57 kDa observed; 52.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cells stimulated with BMP7.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected phospho-Smad1 (Ser463, Ser465) in HepG2 cells stimulated with BMP7.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE2871 04054839337185