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07-164-AF647 Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate

This AlexaFluor®647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Histones, Chromatin Biology Gene Symbol: H2AFX, H2AX UniProt Number: P16104
07-164-AF647
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRbAlexaFluor®647Polyclonal Antibody
Description
Catalogue Number07-164-AF647
DescriptionAnti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate
Alternate Names
  • Histone H2A.X
  • Histone H2A.X
  • H2a/x
Background InformationHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.
References
Product Information
FormatAlexaFluor®647
PresentationPurified conjugated rabbit polyclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis AlexaFluor®647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X.
Key Applications
  • Immunocytochemistry
Application NotesSee data sheet for cat# 07-164, Anti-phospho H2A.X (Ser139) for additional Western blot, Immnoprecipitation, and ChIP-seq analysis performed using an unconjugated version of this antibody.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to phospho H2A.X (Ser139).
EpitopePhosphorylated Ser139
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes Histone H2A.X phosphorylated at Serine 139.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Vertebrates based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • H2AFX
  • H2AX
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~15 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceImmunocytochemistry Analysis:

A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in HeLa cells.
Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-164-AF647 04053252983672

Documentation

Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate Certificates of Analysis

TitleLot Number
Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate 3033499
Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate 2846384
Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - Q2443132 Q2443132
Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 3185032 3185032
Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 3508342 3508342
Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 3819095 3819095
Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 3944550 3944550
Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 4081596 4081596

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Life Science Research > Antibodies and Assays > Primary Antibodies