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AB356481 Anti-mCherry

Detect mCherry protein using this chicken polyclonal anti-mCherry Antibody, Cat. No. AB356481. It has been tested in Immunofluorescence and Western Blotting.

Anti-mCherry MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Gene Symbol: mCherry UniProt Number: Q9U6Y8
AB356481
50 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, IFChUnpurifiedPolyclonal Antibody
Description
Catalogue NumberAB356481
DescriptionAnti-mCherry
Background InformationmCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but it produces a red rather than a green fluorescence. mCherry is a monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, polyclonal was raised in chicken egg and the antibody preparation was dialyzed against phosphate buffered saline. This antibody recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72; Gross, LA et al. (2000). Proc. Natl. Acad. Sci. USA. 97(22):11990-95).
References
Product Information
FormatUnpurified
PresentationChicken polyclonal antibody in PBS with 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect mCherry protein using this chicken polyclonal anti-mCherry Antibody, Cat. No. AB356481. It has been tested in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesImmunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. (Courtesy of Dr Gerry Shaw).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. (Courtesy of Dr Gerry Shaw).
Biological Information
ImmunogenHis-tagged full length recombinant mCherry from Discosoma sp (Mushroom anemone).
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostChicken
SpecificityThis polyclonal antibody detects mCherry in HEK293 cells tranfected with pFin-mCherry.
IsotypeIgY
Species Reactivity
  • All
Species Reactivity NoteAll
Antibody TypePolyclonal Antibody
Gene Symbol
  • mCherry
Purification MethodUnpurified
UniProt Number
Molecular Weight~32 kDa observed; 25.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 transfected with pFin-EF1-mCherry vector.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected mCherry in 10 µg of HEK293 cells transfected with pFin-EF1-mCherry vector.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB356481 04054839189159