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MABS2029-25UG Anti-YAP Antibody, clone 8G5

Anti-YAP, clone 8G5, Cat. No. MABS2029, is a rat monoclonal antibody that detects Transcriptional coactivator YAP1 and has been tested or use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-YAP Antibody, clone 8G5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: YAP1, YAP65 UniProt Number: P46937
MABS2029-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HICC, IF, IH(P), IP, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS2029-25UG
DescriptionAnti-YAP Antibody, clone 8G5
Alternate Names
  • Transcriptional coactivator YAP1
  • Yes-associated protein 1
  • Protein yorkie homolog
  • Yes-associated protein YAP65 homolog
Background InformationTranscriptional coactivator YAP1 (UniProt: P46937; also known as Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog) is encoded by the YAP1 (also known as YAP65) gene (Gene ID: 10413) in human. YAP1 is a transcriptional regulator that can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. It also plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. YAP1 contains two WW domains (aa 171-204 and 230-263) and a transactivation domain (aa 291-504). It also has two coiled coil regions (aa 86-100 and 298-359) and the first coiled-coil region is reported to mediate most of the interaction with TEAD transcription factors. The presence of TEAD transcription factors is required for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. YAP1 phosphorylation status and cell density can regulate its subcellular localization. Its phosphorylation by LATS1 and LATS2 leads to its cytoplasmic translocation and inactivation. YAP1 can also be phosphorylated by ABL1, which leads to its stabilization, increased interaction with TP73, and recruitment onto proapoptotic genes in response to DNA damage. Nine isoforms of YAP1 have been described that are produced by alternative splicing. Mutations in YAP1 gene have been linked to ocular coloboma that is characterized by uveal colobomata, microphthalmia, cataract and cleft lip/palate. Higher expression of YAP1 has been reported in some liver and prostate cancers.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-YAP, clone 8G5, Cat. No. MABS2029, is a rat monoclonal antibody that detects Transcriptional coactivator YAP1 and has been tested or use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected YAP in mouse colon tissue sections.

Western Blotting Analysis: A representative lot detected YAP in Western Blotting applications (Miyamura, N.,et. al. (2017). Nat Commun. 8:16017; Matsudaira, T., et. al. (2017). Nat Commun. 8(1):1246; Hata, S., et. al. (2012). J Biol Chem. 287(26):22089-98; Maruyama, J., et. al. (2017). Mol Cancer Res. 16(2):197-211).

Immunoprecipitation Analysis: A representative lot detected YAP in Immunoprecipitation applications (Hata, S., et. al. (2012). J Biol Chem. 287(26):22089-98).

Immunofluorescence Analysis: A representative lot detected YAP in Immunofluorescence applications (Miyamura, N.,et. al. (2017). Nat Commun. 8:16017).

Immunocytochemistry Analysis: A representative lot detected YAP in Immunocytochemistry applications (Matsudaira, T., et. al. (2017). Nat Commun. 8(1):1246; Hata, S., et. al. (2012). J Biol Chem. 287(26):22089-98).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Transcriptional coactivator YAP1.
EpitopeC-terminus
Clone8G5
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 8G5 is a rat monoclonal antibody that detects Transcriptional coactivator YAP1. It targets an epitope with in 12 amino acids from the C-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • YAP1
  • YAP65
Purification MethodProtein G purified
UniProt Number
Molecular Weight54.46 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected YAP in human placenta tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2029-25UG 04054839519468