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ABE1336-25UG Anti-Wee1

Anti-Wee1 Antibody, Cat. No. ABE1336-25UG, is a highly specific rabbit polyclonal antibody, that targets Wee1-like protein kinase and has been tested in Western Blotting and Immunohistochemistry (Paraffin).

Anti-Wee1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: WEE1 UniProt Number: P30291
ABE1336-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1336-25UG
DescriptionAnti-Wee1
Alternate Names
  • Wee1-like protein kinase
  • EC: 2.7.10.2
  • WEE1hu
  • Wee1A kinase
Background InformationWee1-like protein kinase (UniProt: P30291; also known as EC: 2.7.10.2, WEE1hu, Wee1A kinase) is encoded by the WEE1 gene (Gene ID: 7465) in human. Wee1 is a magnesium-dependent nuclear kinase that belongs to the Serine/Threonine family of protein kinases. It serves as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed Cdk1 prior to the onset of mitosis. Two isoforms of Wee1 have been described that are produced by alternative splicing. In isoform 1 the nucleotide binding site is localized to amino acids 305-313 and its protein kinase domain lies within amino acids 299-569. Wee1 specifically phosphorylates and inactivates cyclin B1-complexed Cdk1, reaching a maximum during G2 phase and a minimum as cells enter into M phase. Phosphorylation of cyclin B1-Cdk1 is shown to occur exclusively on Tyrosine 15. Wee1 levels are believed to increase during S and G2 phases due to increase in transcription and a decline in M phase has been attributed to reduced synthesis and higher degradation. Phosphorylation at serine 642 by BRSK1 and BRSK2 in post-mitotic neurons is reported to down-regulate Wee1 activity in polarized neurons. Also, phosphorylation at serine 53 by PLK1 and at serine 123 by Cdk1 generates a signal for its ubiquitination and proteasomal degradation at the onset of G2/M phase.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Wee1 Antibody, Cat. No. ABE1336-25UG, is a highly specific rabbit polyclonal antibody, that targets Wee1-like protein kinase and has been tested in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected Wee1 in 10 µg of K562 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Wee1 in human placenta and human testis cancer tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Wee1-like protein kinase. The immunogen sequence is conserved between isoforms 1 and 2.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Wee1-like kinase in human cells. It targets an epitope within 15 amino acids from the C-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • WEE1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~72 kDa observed; 71.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Wee1 in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1336-25UG 04054839431685