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ABS82-AF488 Anti-VEGF Antibody, Alexa Fluor® 488 Conjugate

Anti-VEGF Antibody, Alexa Fluor® 488 Conjugate is an antibody against VEGF for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: VEGFA, VEGF UniProt Number: P15692
ABS82-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue NumberABS82-AF488
DescriptionAnti-VEGF Antibody, Alexa Fluor® 488 Conjugate
Alternate Names
  • Vascular endothelial growth factor A
  • VEGF-A
  • Vascular permeability factor
  • VPF
  • VEGF
Background InformationVEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic factor. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties, such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF also enhances permeabilization of blood vessels and plays a central role in the regulation of vasculogenesis. VEGF expression has been linked to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit polyclonal antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-VEGF Antibody, Alexa Fluor® 488 Conjugate is an antibody against VEGF for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in HeLa and A431 cells.
The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.
EpitopeN-terminus.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody recognizes VEGF at the N-terminus.
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat. Predicted to react with Non-human Primate based on 100% sequence homology. Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • VEGFA
  • VEGF
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~55 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in NIH/3T3 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS82-AF488 04055977333442