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MAB5280 Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15

MAB5280
40 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, Qu, RIHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5280
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tyrosine Hydroxylase Antibody, clone 2/40/15
Alternate Names
  • TH
  • Tyrosine Monooxygenase
Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Product Information
FormatPurified
Control
  • Positive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue
PresentationPurified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry:
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.

Western blot:
1:1000-1:2000

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).
Clone2/40/15
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityTyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Chicken
  • Quail
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Gene Symbol
  • TH
  • TYH
  • EC 1.14.16.2
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Molecular Weight56-60 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Packaging Information
Material Size40 µg
Global Trade Item Number
Catalogue Number GTIN
MAB5280 04053252398896