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MABN2406 Anti-Tau Antibody, clone TNT-2

Anti-Tau Antibody, clone TNT-2, Cat. No. MABN2406, is a highly specific mouse monoclonal antibody that targets Tau and has been tested in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-Tau Antibody, clone TNT-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: MAPT, MAPTL, MTBT1, TAU UniProt Number: P10636
MABN2406
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, M, HIF, ELISA, WB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2406
DescriptionAnti-Tau Antibody, clone TNT-2
Alternate Names
  • Microtubule-associated protein tau
  • Neurofibrillary tangle protein
  • Paired helical filament-tau
  • PHF-tau
Background InformationMicrotubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau proteins are mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. Normally, Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The localization of Tau protein in the Alzheimer s disease (AD) brain is markedly abnormal and may contribute to neuronal dysfunction. In AD, normal soluble cytoskeletal elements, such as Tau and neurofilaments, are transformed into insoluble paired helical filaments (PHFs). This is linked to the post-translational change in Tau, primarily its hyperphosphorylation by a number of protein kinases. In its non-phosphorylated state, Tau promotes rapid and extensive microtubule (MT) polymerization. A significant consequence of Tau hyperphosphorylation in AD is a reduction in its ability to bind microtubules and promote microtubule assembly. Hyperphosphorylated Tau may contribute to a destabilized microtubule network, impaired axonal transport, and ultimately result in NFT formation and neuronal death.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Tau Antibody, clone TNT-2, Cat. No. MABN2406, is a highly specific mouse monoclonal antibody that targets Tau and has been tested in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunofluorescence
  • ELISA
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 0.02 µg/mL from a representative lot detected Tau in 10 µg of mouse entorhinal cortex.

Immunohistochemistry Analysis: A representative lot detected Tau in Immunohistochemistry applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

ELISA Analysis: A representative lot detected Tau in ELISA applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

Western Blotting Analysis: A representative lot detected Tau in Western Blotting applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

Immunofluorescence Analysis: A representative lot detected Tau in Immunofluorescence applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of human Tau.
CloneTNT-2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone TNT-2 specirfically detects tau in human, mouse, and rat brain. It targets a sequence of 17 amino acids from the N-terminal region that is common to all isoforms (1-6) of tau.
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MAPT
  • MAPTL
  • MTBT1
  • TAU
Purification MethodProtein G purified
UniProt Number
Molecular Weight~55 kda Observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat entorhinal cortex.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected Tau in 10 µg of rat entorhinal cortex lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2406 04054839174520