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AB5330-50UL Anti-Syntaxin 4 Antibody

AB5330-50UL
50 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5330-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Syntaxin 4 Antibody
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Quality LevelMQ100
Applications
ApplicationAnti-Syntaxin 4 Antibody is an antibody against Syntaxin 4 for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blotting: 1:200-1:500 using ECL on rat kidney membranes.


Dilutions should be made using a carrier protein such as BSA (1-3%).


Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHighly purified corresponding to residues 2-23 of rat or mouse Syntaxin 4 (Accession Q08850).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Syntaxin 4.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • STX4
  • p35-2
  • STX4A
  • Syntaxin-4
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q12846 # Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.
SIZE: 297 amino acids; 34180 Da
SUBUNIT: Binds STXBP3 and STXBP6. Binding to STXBP3 excludes binding with SNAP25. Interacts with SNAP23 and SNAP25BP. Found in a complex with VAMP8 and SNAP23 in pancreas (By similarity). Interacts with VAMP2 and STXBP4 (By similarity). Detected in a complex with SNAP23 and STXBP4. Interaction with STXBP4 excludes interaction with VAMP2 (By similarity). Interacts with STX4A (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type IV membrane protein (Potential).
SIMILARITY: SwissProt: Q12846 ## Belongs to the syntaxin family. & Contains 1 t-SNARE coiled-coil homology domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5330-50UL 04053252678080

Documentation

Anti-Syntaxin 4 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Syntaxin 4 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-SYNTAXIN 4 - 3776078 3776078
RABBIT ANTI-SYNTAXIN 4 -2632540 2632540
RABBIT ANTI-SYNTAXIN 4 -2848185 2848185
RABBIT ANTI-SYNTAXIN 4 -2860205 2860205
RABBIT ANTI-SYNTAXIN 4 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3015850
RABBIT ANTI-SYNTAXIN 4 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 1999457 1999457
RABBIT ANTI-SYNTAXIN 4 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2015564 2015564
RABBIT ANTI-SYNTAXIN 4 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2062318 2062318

Data Sheet

Title
RABBIT ANTI-SYNTAXIN 4 AFFINITY PURIFIED POLYCLONAL ANTIBODY