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MABN1101 Anti-SRY, clone 1G4 Antibody

Anti-SRY, clone 1G4 Antibody is an antibody against SRY for use in western blotting, IHC, flow cytometry & ELISA.

Anti-SRY, clone 1G4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: SRY, TDF UniProt Number: Q05066
MABN1101
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, FC, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1101
DescriptionAnti-SRY, clone 1G4 Antibody
Alternate Names
  • Sex-determining region Y protein
  • Testis-determining factor
  • SRY
Background InformationSex-determining region Y protein, also known as Testis-determining factor, and encoded by the human gene SRY or TDF is a transcriptional regulator that controls a genetic switch in male development. SRY is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) to Sertoli rather than granulosa cells. In male adult brain SRY is involved in the maintenance of motor functions of dopaminergic neurons. SRY is also involved in different aspects of gene regulation including promoter activation or repression, DNA bending and manipulation. The SRY HMG box recognizes DNA by partial intercalation into the minor groove, and SRY is also involved in pre-mRNA splicing. SRY is expressed in the developing testis and surrounding tissue and can be found expressed in neurons in the adult. SRY is nuclear associated.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal in PBS containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SRY, clone 1G4 Antibody is an antibody against SRY for use in western blotting, IHC, flow cytometry & ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Flow Cytometry
  • ELISA
Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SRY in cervical and ovarian cancer tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SRY in HepG2 cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human SRY expressed in E. Coli
Clone1G4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • SRY
  • TDF
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~36 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NTERA-2 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SRY in NTERA-2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1101 04053252957352