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ABE571 Anti-SOX9 Antibody

ABE571
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE571
DescriptionAnti-SOX9 Antibody
Alternate Names
  • Transcription factor SOX-9
Background InformationSox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SOX9 Antibody is a highly specific rabbit polyclonal antibody, that targets SOX9 & has been tested in western blotting, IHC & Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A representative lot from an independent laboratory detected SOX9 in K14 mouse backskin tissue (Nowak, J. A., et al. (2008). Cell Stem Cell. 3(1):33-43.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected SOX9 in mouse Label Retaining cells, and developing epithelial skin and hair follicle cells (Nowak, J. A., et al. (2008). Cell Stem Cell. 3(1):33-43.).
Biological Information
ImmunogenLinear peptide corresponding to the C-terminus of human SOX9.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Mouse
Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SOX9
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~63 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SOX9 in 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE571 04053252929052