Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

07-1818 Anti-SKAR Antibody

Detect SKAR with Anti-SKAR Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.

Anti-SKAR Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Binding Protein (RBP)
07-1818
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBRbSerumPolyclonal Antibody
Description
Catalogue Number07-1818
DescriptionAnti-SKAR Antibody
Background InformationThe mammalian target of rapamycin (mTOR) and phosphatidylinositol 3-kinase (PI3K) signaling pathways promote cell growth and cell cycle progression in response to nutritional, energy, and mitogenic cues. In mammalian cells, the ribosomal protein S6 kinases, S6K1 and S6K2, lie downstream of mTOR and PI3K, suggesting that translational control through the phosphorylation of S6 regulates cell growth. SKAR is a specific binding partner and substrate of S6K1 but not S6K2. Serines 383 and 385 of human SKAR are insulin-stimulated and rapamycin-sensitive S6K1 phosphorylation sites. SKAR is nuclear protein with homology to the Aly/REF family of RNA binding proteins, which has been proposed to couple transcription with pre-mRNA splicing and mRNA export (Richardson, C.J., 2004).
References
Product Information
FormatSerum
Control
  • HeLa cell lysate
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Applications
ApplicationDetect SKAR with Anti-SKAR Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated synthetic linear peptide corresponding to SKAR.
HostRabbit
SpecificityThis antibody detects SKAR.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. This protein is a specific target of S6 kinase 1 and regulates cell growth. Two transcript variants that encode different protein isoforms have been identified.
Purification MethodUnpurified
Molecular Weight~46-50 kDa.
Non-specific bands may be detected at ~40 kDa and ~200 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by western blot on HeLa cell lysate.
Western Blot Analysis: 1:1000 dilution of this antibody detected SKAR in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1818 04053252471742

Documentation

Anti-SKAR Antibody Certificates of Analysis

TitleLot Number
Anti-SKAR - NG1619909 NG1619909