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ABF125 Anti-S100A8/MRP-8 Antibody (Azide Free)

This Anti-S100A8/MRP-8 Antibody (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Activity Assay for the detection of S100A8/MRP-8 (Azide Free).

Anti-S100A8/MRP-8 Antibody (Azide Free) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: S100a8, Caga, Mrp8 UniProt Number: P27005
ABF125
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHC, ACTRbSerumPolyclonal Antibody
Description
Catalogue NumberABF125
DescriptionAnti-S100A8/MRP-8 Antibody (Azide Free)
Alternate Names
  • Protein S100-A8
  • Calgranulin-A
  • Chemotactic cytokine CP-10
  • Leukocyte L1 complex light chain
  • Migration inhibitory factor-related protein 8
  • MRP-8
  • p8
  • Pro-inflammatory S100 cytokine
  • S100 calcium-binding protein A8
  • S100A8/MRP-8
Background InformationS100A8, also known as Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, Migration inhibitory factor related protein 8 (MRP-8), p8, S100 calcium binding protein A8, or Urinary stone protein band A, and encoded by the gene S100A8/CAGA/CFAG/MRP8, is an important protein that binds both zinc and calcium and plays an important role in the regulation of inflammatory processes and immune response. Found in complex with its partner S100A9, S100A8 facilitates chemotaxis, fatty acid trafficking, cytoskeleton reorganization, and NAPDPH oxidase activation and other intracellular activities, as well as a host of extracellular induced activities such as extracellular proinflammatory, antimicrobial, oxidant scavenging, and apoptotic activities . Additionally S100A8 can act as a potent autoimmunity amplifier and appears to augment various cancers and their spread when over expressed. S100A8 is widely expressed and used as biomarker in patients with inflammatory diseases and as a cancer marker in multiple forms of cancer.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-S100A8/MRP-8 Antibody (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Activity Assay for the detection of S100A8/MRP-8 (Azide Free).
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Activity Assay
Application NotesActivity Assay Analysis: A representative lot detected that S100A8/MRP-8 reduces mouse neutrophil accumulation in dorsum-raised air pouchs in the presence of MSU crystals (Ryckman, C., et al. (2003). ARTHRITIS & RHEUMATISM. 48(8):2310–2320).
Immunohistochemistry Analysis: A representative lot detected S100A8/MRP-8 in mouse arthritic paw tissue (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e4578).
Biological Information
ImmunogenRecombinant protein corresponding to mouse S100A8/MRP-8.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • S100a8
  • Caga
  • Mrp8
Purification MethodUnpurified
UniProt Number
Molecular Weight~10 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected S100A8/MRP-8 in 10 µg of human spleen tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABF125 04053252983405

Documentation

Anti-S100A8/MRP-8 Antibody (Azide Free) SDS

Title

Safety Data Sheet (SDS) 

Anti-S100A8/MRP-8 Antibody (Azide Free) Certificates of Analysis

TitleLot Number
Anti-S100A8/MRP-8 (Azide Free) - Q2441541 Q2441541
Anti-S100A8/MRP-8 (Azide Free) - 3587082 3587082

References

Reference overviewPub Med ID
Role of S100A8 and S100A9 in neutrophil recruitment in response to monosodium urate monohydrate crystals in the air-pouch model of acute gouty arthritis.
Ryckman, Carle, et al.
Arthritis Rheum., 48: 2310-20 (2003)  2003

Show Abstract 		12905486 12905486