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NR07 Anti-Ryanodine Receptor Mouse mAb (C3-33)

This Anti-Ryanodine Receptor Mouse mAb (C3-33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation for the detection of Ryanodine Receptor.

Anti-Ryanodine Receptor Mouse mAb (C3-33) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NR07
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Am, Av, Ca, F, Rb, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
NR07-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~565 kDa ryanodine receptor in rat brain hippocampus. A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates.
Catalogue NumberNR07
Brand Family Calbiochem®
Application Data

Detection of rat ryanodine receptor by immunocytochemistry. Sample: Rat brain hippocampus. Primary antibody: Anti-Ryanodine Receptor Mouse mAb (C3-33) (Cat. No. NR07) (1:50). Detection: fluorescence.
References
ReferencesSitsapesan, R., et al. 1995. Trends Pharmacol. Sci. 16, 386.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem. 268, 13765.
Meszaros, L.G., et al. 1993. Nature 364, 76.
Lai, F.A., et al. 1992. Biochem. J. 288, 553.
Lai, F.A., et al. 1989. Nature 331, 315.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlRat brain hippocampus
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Lai, F.A., et al. 1992. Amer. J. Physiol. 263, C365. Frozen Sections Junker, J., et al. 1994. J. Biol. Chem. 269, 1627.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesFrozen Sections (1 µg/ml, see application references)
Immunoblotting (1 µg/ml)
Immunoprecipitation (see comments)
Application CommentsA ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogencanine cardiac ryanodine receptor (RYR)
ImmunogenCanine
CloneC3-33
HostMouse
IsotypeIgG₁
Species Reactivity
  • Amphibian
  • Avian
  • Canine
  • Fish
  • Rabbit
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NR07-100UG 04055977210002

Documentation

Anti-Ryanodine Receptor Mouse mAb (C3-33) SDS

Title

Safety Data Sheet (SDS) 

Anti-Ryanodine Receptor Mouse mAb (C3-33) Certificates of Analysis

TitleLot Number
NR07

References

Reference overview
Sitsapesan, R., et al. 1995. Trends Pharmacol. Sci. 16, 386.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem. 268, 13765.
Meszaros, L.G., et al. 1993. Nature 364, 76.
Lai, F.A., et al. 1992. Biochem. J. 288, 553.
Lai, F.A., et al. 1989. Nature 331, 315.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-July-2018 JSW
ApplicationFrozen Sections (1 µg/ml, see application references)
Immunoblotting (1 µg/ml)
Immunoprecipitation (see comments)
Application Data

Detection of rat ryanodine receptor by immunocytochemistry. Sample: Rat brain hippocampus. Primary antibody: Anti-Ryanodine Receptor Mouse mAb (C3-33) (Cat. No. NR07) (1:50). Detection: fluorescence.
DescriptionMouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X 63 AG8.653 mouse myeloma cells (see application references). Supplied as protein G purified antibody. Recognizes the ~565 kDa ryanodine receptor.
BackgroundPrecise regulation of the intracellular calcium level plays a critical role in numerous brain functions. The identification of intracellular calcium-release channels was aided by the biochemical use of ryanodine, a neutral plant alkaloid known to effect calcium release from the sarcoplasmic reticulum. Ryanodine receptors were originally identified in the sarcoplasmic reticulum of skeletal and cardiac muscle. Three distinct receptor subtypes have since been characterized and include RyR1, RyR2, and RyR3. RyR1 is predominantly expressed in skeletal muscle, but is also localized to Purkinje cells in the cerebellum. RyR2 is primarily expressed in the heart, although expression has been localized to stomach, endothelial cells, and the brain. RyR2 shows widespread neuronal distribution, and is the primary form of the ryanodine receptor expressed in the brain. RyR3 is expressed in the striatum, thalamus, and hippocampus of the brain, as well as smooth muscle and epithelial cells. In non-skeletal muscle, several agonists have been proposed to open the RyR, including calcium or cADP-ribose. The distribution of the ryanodine receptor within the mammalian central nervous system is overlapping but distinct from the inositol 1,4,5-triphosphate receptors (IP3Rs) which are also involved in intracellular Ca2+ release.
HostMouse
Immunogen speciesCanine
Immunogencanine cardiac ryanodine receptor (RYR)
CloneC3-33
IsotypeIgG₁
Speciesamphibian, avian, canine, fish, rabbit (weakly), rat
Positive controlRat brain hippocampus
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsA ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesSitsapesan, R., et al. 1995. Trends Pharmacol. Sci. 16, 386.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem. 268, 13765.
Meszaros, L.G., et al. 1993. Nature 364, 76.
Lai, F.A., et al. 1992. Biochem. J. 288, 553.
Lai, F.A., et al. 1989. Nature 331, 315.
Application referencesOriginal Clone Lai, F.A., et al. 1992. Amer. J. Physiol. 263, C365. Frozen Sections Junker, J., et al. 1994. J. Biol. Chem. 269, 1627.