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05-530-I-25UG Anti-Rad51 Antibody, clone 3C10 (mouse monoclonal)

05-530-I-25UG
25 μg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information
Description
Catalogue Number05-530-I-25UG
DescriptionAnti-Rad51 Antibody, clone 3C10 (mouse monoclonal)
Alternate Names
  • DNA repair protein RAD51 homolog 1
  • HsRAD51
  • hRAD51
  • RAD51 homolog A
  • Rad51
Background InformationDNA repair protein RAD51 homolog 1 (UniProt Q06609; also known as BRCA1/BRCA2-containing complex subunit 5, Homolog of E. coli RecA, hRAD51, HsRAD51, RAD51 homolog A, RecA-like protein, Recombination protein A) is encoded by the RAD51 (also known as HRAD51, HsT16930, BRCC5, MRMV2, RAD51A, RECA) gene (Gene ID 5888) in human. RAD51 is a member of the RecA family recombinases. It interacts with BRCA1 and BRCA2 to activate homologous recombination. RAD51 exhibits DNA-dependent ATPase activity and participates in double-strand break (DSB) repair by unwinding DNA to form a helical nucleoprotein filament. It is highly expressed in the testis and thymus and lower expression levels are found in the small intestine, placenta, colon, pancreas, and ovaries.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Rad51 Antibody, clone 3C10 is validated for use in Western Blotting, Immunoprecipitation for the detection of Rad51 .
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: Reported by an independent laboratory to immunoprecipitate.
Biological Information
ImmunogenRecombinant protein corresponding to human Rad51.
Clone3C10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAD51
  • RAD51A
  • RECA
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~37 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rad51 in 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-530-I-25UG 04054839342387

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