Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2435-25UG Anti-Peripherin-2 Antibody, clone 5H2

Anti-Peripherin-2, clone 5H2, Cat. No. MABN2435, is a highly specific mouse monoclonal antibody that targets Peripherin-2 and has been tested in Immunofluorescence, Radioimmunoassay, and Western Blotting.

Anti-Peripherin-2 Antibody, clone 5H2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: PRPH2, RDS UniProt Number: P17810
MABN2435-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, BIF, RIA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2435-25UG
DescriptionAnti-Peripherin-2 Antibody, clone 5H2
Alternate Names
  • Retinal degeneration slow protein
Background InformationPeripherin-2 (UniProt: P17810; also known as Retinal degeneration slow protein) is encoded by the PRPH2 (also known as RDS) gene in bovine species. Peripherin-2 is a disulfide-linked homodimeric, multi-pass membrane protein that may function as an adhesion molecule involved in stabilization and compaction of outer segment disks in retina and is considered to be essential for disk morphogenesis. It is exclusively expressed in outer segments. Peripherin-2 can form homo- and heteromeric protein complexes in outer segment. The core homomeric peripherin-2 unit is the non-covalent tetramer, which can also assemble into covalently linked octamers and higher-order oligomers. The homomeric peripherin-2 complexes are essential for proper outer segment morphology and architecture. Peripherin-2 is also shown to form heteromeric complexes with its homolog ROM-1. Mutations in PRPH2 gene can lead to autosomal dominant retinitis pigmentosa (adRP) And a majority of these mutations are located within the large loop domain connecting the transmembrane domain 3 and 4 (Ref.: Bohm, S et al. (2017). Scientific Reports 7, Article number: 2321).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Peripherin-2, clone 5H2, Cat. No. MABN2435, is a highly specific mouse monoclonal antibody that targets Peripherin-2 and has been tested in Immunofluorescence, Radioimmunoassay, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Radioimmunoassay
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Peripherin-2 in Western Blotting applications (Connell, G., et. al. (1991). Proc Natl Acad Sci USA. 88(3):723-6; Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).

Radioimmunoassay Analysis: A representative lot detected Peripherin-2 in Radioimmunoassay applications (Connell, G., et. al. (1991). Proc Natl Acad Sci USA. 88(3):723-6).

Immunofluorescence Analysis: A representative lot detected Peripherin-2 in Immunoflourescence applications (Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).
Biological Information
ImmunogenPurified bovine whole peripherin.
Clone5H2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5H2 detects Peripherin-2 in bovine and mouse retina.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Bovine
Species Reactivity NoteBovine, Mouse.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PRPH2
  • RDS
Purification MethodProtein G purified
UniProt Number
Molecular Weight~39 kDa observed; 38.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in bovine retinal tissue lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected Peripherin-2 in 10 µg of bovine retinal tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2435-25UG 04054839522130