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ABT288-25UL Anti-PICALM

Anti-PICALM Antibody, Cat. No. ABT288, is a rabbit polyclonal ntibody that detects PICALM in nhuman cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PICALM MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: PICALM, CALM UniProt Number: Q13492
ABT288-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT288-25UL
DescriptionAnti-PICALM
Alternate Names
  • Phosphatidylinositol-binding clathrin assembly protein
  • Clathrin assembly lymphoid myeloid leukemia protein
Background InformationPhosphatidylinositol-binding clathrin assembly protein (UniProt: Q13492; also known as PICALM, Clathrin assembly lymphoid myeloid leukemia protein) is encoded by the PICALM (also known as CALM) gene (Gene ID: 8301) in human. PICALM is ubiquitously expressed protein that co-localizes with clathrin in the Golgi area. Five isoforms of PICALM have been reported that are produced by alternative splicing. PICALM acts as an assembly protein that recruits clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. It may also play a role in determining the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. It is reported to be involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. PICALM variants are associated with Alzheimer's disease and are thought to be involved in the endocytosis and recycling of the amyloid protein precursor and it can affect Alzheimer s disease disk primarily by modulating production, transportation, and clearance of beta-amyloid peptides. (Ref.: Xu, W., et al. (2015). Mol Neurobiol. 52(1);399-413).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PICALM Antibody, Cat. No. ABT288, is a rabbit polyclonal ntibody that detects PICALM in nhuman cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected PICALM in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PICALM in MOG-G-UVW human astrocytoma cell line (Courtesy of Dr Rhian Thomas from Cardiff University).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot PICALM in Mog-G-UVW human astrocytoma cell lysate and HeLa cell lysate (Courtesy of Dr Rhian Thomas from Cardiff University).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PICALM in Human H4 neuroglioma cell lysate and SH-SY5Y neuroblastoma cell lysate (Courtesy of Dr Rhian Thomas from Cardiff University).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected PICALM in human colon and human kidney tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Phosphatidylinositol-binding clathrin assembly protein (PICALM).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Phosphatidylinositol-binding clathrin assembly protein (PICALM) in human cells. it targets an epitope within 16 amino acids from the C-terminal half. It recognizes all 5 isoforms of PICALM.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Gene Symbol
  • PICALM
  • CALM
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~61 kDa observed; 70.76, 68.76, 66.39, 59.92, and 70.04 kDa calculated, respectively for isoforms 1 to 5. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PICALM in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT288-25UL 04054839512469