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CBL1337F Anti-PECAM-1 Antibody, clone 390, FITC conjugated

CBL1337F
500 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, IHCRFITCMonoclonal Antibody
Description
Catalogue NumberCBL1337F
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-PECAM-1 Antibody, clone 390, FITC conjugated
Alternate Names
  • CD31
Background InformationPECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
Product Information
FormatFITC
PresentationPurified immunoglobulin conjugated to fluorescein. Liquid in in phosphate buffered saline containing 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-PECAM-1 Antibody, clone 390, FITC conjugated is validated for use in FC, IH for the detection of PECAM-1.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
Application NotesFlow Cytometry: = 1 μg per 10e6 cells in 100 μL volume

Immunohistochemistry: acteone fixed frozen tissue sections.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenMouse leukocyte cell line 32D
Clone390
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
SpecificityCBL1337 recognizes mouse PECAM-1, a 130-140 kDa type 1 integral membrane glycoprotein. This antibody has been shown to inhibit the aggregation of L cells transfected with a variant form of PECAM-1 (DeLisser, 1993).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PECAM1
  • EndoCAM
  • GPIIA
  • PECAM-1
  • CD31
  • CD31/EndoCAM
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.
PTM: Phosphorylated on Ser and Tyr residues after cellular activation.
SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2°-8°C for up to 12 months from date of receipt. DO NOT FREEZE.
Packaging Information
Material Size500 µg
Global Trade Item Number
Catalogue Number GTIN
CBL1337F 04053252278334