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MABC1122-100UG Anti-PD-1 Antibody, clone EH33

Anti-PD-1, clone EH33, Cat. No. MABC1122, is a mouse monoclonal antibody that detects Programmed cell death protein 1 (PD-1) in human cells and has been tested for use in Immunohistochemistry (Paraffin).

Anti-PD-1 Antibody, clone EH33 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: PDCD1, PD1 UniProt Number: Q15116
MABC1122-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1122-100UG
DescriptionAnti-PD-1 Antibody, clone EH33
Alternate Names
  • Programmed cell death protein 1
  • hPD-1
  • CD279
Background InformationProgrammed cell death protein 1 (UniProt: Q15116; also known as Protein PD-1, hPD-1, CD279) is encoded by the PDCD1 (also known as PD1) gene (Gene ID: 5133) in human. PD-1 is a monomeric inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. PD-1 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 24-170), a transmembrane domain (aa 171-191), and a cytoplasmic domain (aa 192-288). PD-1 also contains a single N-terminal immunoglobulin variable region (IgV) like domain. PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity. The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells. PD-1 lacks SH2- or SH3-binding motifs on its cytoplasmic tail, but contains the N-terminal sequence VDYGEL that forms an immunoreceptor tyrosine-based inhibition motif (V/I/LxYxxL), which recruits SH2 domain-containing phosphatases. The cytoplasmic tail also contains the C-terminal sequence TEYATI, which forms an immunoreceptor tyrosine-based switch motif (TxYxxL). PD-1 ligation is reported to inhibit the activation of T-cell receptor proximal kinases, which results in attenuation of Lck-mediated phosphorylation of ZAP-70 and initiation of downstream events. PD-1 is also reported to impair the activation of the MEK-ERK MAP kinase pathway by inhibiting activation of PLC- 1 and Ras. (Ref.: Boussiotis, VA (2016). N. Engl. J. Med. 375; 1767-1778).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PD-1, clone EH33, Cat. No. MABC1122, is a mouse monoclonal antibody that detects Programmed cell death protein 1 (PD-1) in human cells and has been tested for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A representative lot detected PD-1 in Immunohistochemistry applications (Sridharan, V., et. al. (2016). Cancer Immunol Res. 4(8):679-87; Calles, A., et. al. (2015). J Thorac Oncol. 10(12):1726-35; Bachireddy, P., et. al. (2014). Blood. 123(9):1412-21).
Biological Information
ImmunogenRecombinant protein fragment corresponding to the extracellular domain of human PD-1 (as an Ig fusion protein).
Epitopeextracellular domain
CloneEH33.1F9.G4.5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone EH33 specifically detects PD-1 in human cells. It targets an epitope with the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PDCD1
  • PD1
Purification MethodProtein G purified
UniProt Number
Molecular Weight31.65 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human tonsil and human bone marrow tissues.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected PD-1 in human tonsil and human bone marrow tissues.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1122-100UG 04054839448317