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ABT1384-25UL Anti-Neurocan Antibody, N-terminal

Anti-Neurocan, N-terminal, Cat. No. ABT1384, is a rabbit polyclonal antibody that detects Neurocan core protein and has been tested for use in Immunofluorescence, Immunohistochemistry, and Radioimmunoassay.

Anti-Neurocan Antibody, N-terminal MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Ncan, Cspg3 UniProt Number: P55067
ABT1384-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RIF, IHC, RIARbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABT1384-25UL
DescriptionAnti-Neurocan Antibody, N-terminal
Alternate Names
  • Neurocan core protein
  • 245 kDa early postnatal core glycoprotein
  • Chondroitin sulfate proteoglycan 3
Background InformationNeurocan core protein (UniProt: P55067; also known as 245 kDa early postnatal core glycoprotein, Chondroitin sulfate proteoglycan 3) is encoded by the Ncan (also known as Cspg3) gene in rat. Neurocan is a secreted protein of the aggrecan/versican proteoglycan family that is found in early postnatal and adult brain. During early postnatal development neurocan accounts for at least 20% of the protein of the soluble brain proteoglycans. Neurocan mostly contains chondroitin sulfate, but also has N-linked and O-linked oligosaccharides. It is shown to be involved in the modulation of cell adhesion and migration and regulates neurite outgrowth. Neurocan interact with several cell surface molecules, such as N-CAM, L1/Ng-CAM, etc., and modulates the cell-binding and neurite outgrowth promoting activates of these molecules. Neurocan is synthesized with a 22 aa signal peptide that is subsequently cleaved to produce the mature form. Neurocan contains one C-type lectin domain, two EGF-like domains, one Ig-like V type domain, two link domains and one Sushi (CCP/SCR) domain. (Ref.: Rauch, U., et al. (2001) Cell Mol. Life Sci. 58(12-13):1842-56; Zhou, XH et al. (2001). Mol. Cell Biol. 21(17): 5970-78).
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-Neurocan, N-terminal, Cat. No. ABT1384, is a rabbit polyclonal antibody that detects Neurocan core protein and has been tested for use in Immunofluorescence, Immunohistochemistry, and Radioimmunoassay.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Radioimmunoassay
Application NotesRadioimmunoassay Analysis: A representative lot detected Neurocan in rat brain (Milev, P., et. al. (1998). Biochem Biophys Res Commun. 247(2):207-12).

Immunohistochemistry Analysis: A representative lot detected Neurocan in E13 and E14 mouse ventral diencephalon and retina in C57 mouse embryos (Leung, K.M., et. al. (2004). Brain Res Dev Brain Res. 152(1):1-10).
Biological Information
ImmunogenGST-tagged recombinant fragement corresponding to 164 amino acids from the N-terminal region of rat Neurocan.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Neurocan in fetal and adult rat brain.
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat.
Antibody TypePolyclonal Antibody
Gene Symbol
  • Ncan
  • Cspg3
Purification MethodUnpurified
UniProt Number
Molecular Weight135.55 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunofluorescence in E14 Embryonic rat brain (frozen section), rat E14 neocortex, and rat hippocampus cells.

Immunofluorescence Analysis: A 1:500 dilution of this antibody detected Neurocan in E14 Embryonic rat brain (frozen section), rat E14 neocortex, and rat hippocampus cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT1384-25UL 04054839512414

Documentation

Anti-Neurocan Antibody, N-terminal Certificates of Analysis

TitleLot Number
Anti-Neurocan, N-terminal 3072416