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MABF1968-25UG Anti-Microglobulin beta2 Antibody, clone BBM.1

This mouse monoclonal Anti-Microglobulin beta2, clone BBM.1 Antibody, Cat. No. MABF1968-25UG is validated for use in Western Blotting and Immunohistochemistry (Paraffin), Inhibition, and Flow Cytometry, for the detection of Microglobulin beta2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: B2M, CDABP0092, HDCMA22P UniProt Number: P61769
MABF1968-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P), Inhibition, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1968-25UG
DescriptionAnti-Microglobulin beta2 Antibody, clone BBM.1
Alternate Names
  • B2M
Background InformationBeta-2-microglobulin (UniProt: P61769) is encoded by the B2M (also known as CDABP0092, HDCMA22P) gene (Gene ID: 567) in human. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC) that is involved in the presentation of peptide antigens to the immune system. It is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved to generate the mature form. It also contains an Ig-like C1 type domain (aa 25-113). Beta-2-microglobulin lacks a transmembrane anchoring domain and is found in secreted as well as in complex. Serum beta-2-microglobulin levels are a reflection of cell turnover and its levels are shown to be elevated in fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin's lymphoma. Its levels may be elevated either due to higher rates of synthesis as observed in HIV infection and malignancy or due to impaired renal filtration due to renal insufficiency.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationThis mouse monoclonal Anti-Microglobulin beta2, clone BBM.1 Antibody, Cat. No. MABF1968-25UG is validated for use in Western Blotting and Immunohistochemistry (Paraffin), Inhibition, and Flow Cytometry, for the detection of Microglobulin beta2.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Inhibition
  • Flow Cytometry
Application NotesImmunohistochemistry Analysis: A representative lot detected Microglobulin beta2 in Immunohistochemistry applications (Josson, S., et. al. (2011). Cancer Res. 71(7):2600-10).

Inhibition Analysis: A representative lot inhibited Microglobulin beta2 activity (Novoselov, A.V., et. al. (2012). Virus Res. 166(1-2):87-96; Parham, P., et. al. (1983). J Biol Chem. 258(10):6179-86).

Flow Cytometry Analysis: A representative lot detected Microglobulin beta2 in Flow Cytometry applications (Drukker, M., et. al. (2012). Nat Biotechnol. 30(6):531-42).

Immunohistochemistry Analysis: A 1:50-1:1,000 dilution from a representative lot detected Microglobulin beta2 in human breast cancer and human tonsil tissues.
Biological Information
ImmunogenMOLT-4, a Human acute lymphoblastic leukemia cell line.
CloneBBM.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone BBM.1 detects Beta-2-microglobulin in human.
IsotypeIgG2b
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • B2M
  • CDABP0092
  • HDCMA22P
Purification MethodProtein G purified
UniProt Number
Molecular Weight14 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MOLT-4 cell lysates
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Microglobulin beta2 in 10 µg of MOLT-4 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1968-25UG 04054839456176