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MABN699 Anti-Metadherin Antibody, clone 2F11C3

Detect Metadherin using this mouse monoclonal antibody, Anti-Metadherin Antibody, clone 2F11C3 validated for use in western blotting, IHC & Flow Cytometry.

Anti-Metadherin Antibody, clone 2F11C3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: 3D3, AEG1, LYRIC, MTDH UniProt Number: Q86UE4
MABN699
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, FCMAffinity PurifiedMonoclonal Antibody
Description
Catalogue NumberMABN699
DescriptionAnti-Metadherin Antibody, clone 2F11C3
Alternate Names
  • Protein LYRIC
  • 3D3/LYRIC
  • Astrocyte elevated gene-1 protein
  • AEG-1
  • Lysine-rich CEACAM1 co-isolated protein
  • Metadherin
  • Metastasis adhesion protein
Background InformationMetadherin, or more commonly known as LYRIC, or AEG-1 (Astrocyte elevated gene-1) is a single pass membrane protein that plays a key role in anchorage-independent growth of immortalized melanocytes and astrocytes. Anchorage-independent division is a critical component in tumor cell expansion and metastasis. Similarly, metadherin activates the nuclear factor kappa- B (NF-kappa-B) transcription leading to expression of numerous genes involved in cell growth and expansion, and it is also involved in HIF-1alpha mediated angiogenesis in tumor growth. Finally new research demonstrates that metadherin interacts with RNA-induced silencing complex (RISC) and plays a very important role in RISC and miRNA functions as well. The protein is a key target of research because of its tumor promoting effects in lung, bone and brain cancers. Metadherin is localized to the endoplasmic reticulum and nuclear membranes in normal tissue, thus staining is perinuclear primarily but Metadherin staining can also be seen in the nucleus, at tight junctions and even at the cell surface in certain cancers. Metadherin expression is activated by TNF, and metadherin is expressed in many cells types with the highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Metadherin is often over expressed in various cancers including breast, brain, prostate, and melanoma and glioblastoma multiforme. EMD-Millipore’s Anti-metadherin mouse monoclonal, clone 2F11C3 has been tested by western blot against several human cancer cell lysates, by flow cytometry against human HeLa cells, and in human paraffin-embedded human liver tissue.
References
Product Information
FormatAffinity Purified
Control
  • K562 cell lysate
PresentationPurified mouse monoclonal IgG1 in PBS containing up to 0.1% sodium azide and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationDetect Metadherin using this mouse monoclonal antibody, Anti-Metadherin Antibody, clone 2F11C3 validated for use in western blotting, IHC & Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Flow Cytometry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Metadherin in human breast cancer tissue.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected Metadherin in HeLa cells

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified protein fragment of Human Metadherin expressed in E. coli.
Clone2F11C3
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • 3D3
  • AEG1
  • LYRIC
  • MTDH
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~70 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Metadherin in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN699 04053252912382

Documentation

Anti-Metadherin Antibody, clone 2F11C3 SDS

Title

Safety Data Sheet (SDS) 

Anti-Metadherin Antibody, clone 2F11C3 Certificates of Analysis

TitleLot Number
Anti-Metadherin, clone 2F11C3 - QVP1304111 QVP1304111
Anti-Metadherin, clone 2F11C3 - VP1901180 VP1901180
Anti-Metadherin, clone 2F11C3 -VP1504145 VP1504145
Anti-Metadherin, clone 2F11C3 -VP1506293 VP1506293
Anti-Metadherin, clone 2F11C3 -VP1510270 VP1510270
Anti-Metadherin, clone 2F11C3 -VP1510294 VP1510294
Anti-Metadherin, clone 2F11C3 -VP1603023 VP1603023
Anti-Metadherin, clone 2F11C3 -VP1610132 VP1610132