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AB5675-AF488 Anti-Metabotropic Glutamate Receptor 5 Antibody, Alexa Fluor® 488

Anti-Metabotropic Glutamate Receptor 5 Antibody, Alexa Fluor® 488 is an antibody against Metabotropic Glutamate Receptor 5 for use in Immunocytochemistry, Immunofluorescence.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: Grm5, Gprc1e, Mglur5 UniProt Number: Q3UVX5
AB5675-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RICC, IFRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue NumberAB5675-AF488
DescriptionAnti-Metabotropic Glutamate Receptor 5 Antibody, Alexa Fluor® 488
Alternate Names
  • Metabotropic glutamate receptor 5
  • mGluR5
  • G protein-coupled receptor GRM5
  • G protein coupled receptor family C, group 1, member E
Background InformationMetabotropic glutamate receptor 5 or mGluR5 (UniProt P31424; also known as G protein-coupled receptor GRM5, G protein coupled receptor family C, group 1, member E) is encoded by the Grm5 (also known as Gprc1e, Mglur5) gene (Gene ID 24418) in rat species. The metabotropic glutamate receptors belong to a family of G protein-coupled receptors that have been divided into 3 groups based on their sequence homology, putative signal transduction mechanisms, and pharmacologic properties. mGluR1 and mGluR5 constitute Group I metabotropic glutamate receptors. mGluR5 is found on postsynaptic terminals of neurons and microglia. mGluR5 signals via Gq/G11 coupling to activate phospholipase C, resulting in calcium mobilization and activation of protein kinase C (PKC). In addition, mGluR5 is also reported to mediate signaling involving Src-family protein tyrosine kinase in a G protein-independent manner.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Metabotropic Glutamate Receptor 5 Antibody, Alexa Fluor® 488 is an antibody against Metabotropic Glutamate Receptor 5 for use in Immunocytochemistry, Immunofluorescence.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
Application NotesImmunocytochemistry Analysis: An 1:100 dilution from a representative lot detected Metabotropic Glutamate Receptor 5 in rat cortical neurons.
The unconjugated antibody (Cat. No. AB5675) is shown to be suitable also for Western blotting and immunohistochemistry applications.
Biological Information
ImmunogenSynthetic peptide corresponding to a sequence from the C-terminal cytoplasmic domain of rat mGluR5.
EpitopeC-terminal cyoplasmic domain.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with all spliced isoforms of human, rat, and mouse mGluR5 reported by UniProt (P41594, P31424 and Q3UVX5, respectively).
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Mouse, Human, Bovine, Primate, Feline, Horse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Grm5
  • Gprc1e
  • Mglur5
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~150 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunofluorescence in rat brain tissue sections.

Immunofluorescence Analysis: An 1:100 dilution of this antibody detected Metabotropic Glutamate Receptor 5 in rat cerebellum and rat brain tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5675-AF488 04055977329230