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AB6004 Anti-MT1-MMP Antibody, hinge region

AB6004
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB6004
ReplacesAB815
DescriptionAnti-MT1-MMP Antibody, hinge region
Alternate Names
  • MT-MMP 1
  • Membrane-type matrix metalloproteinase 1
  • Membrane-type-1 matrix metalloproteinase
  • matrix metallopeptidase 14 (membrane-inserted)
  • matrix metalloproteinase 14
  • matrix metalloproteinase 14 (membrane-inserted)
  • membrane type 1 metalloprotease
Background InformationMatrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade the components of extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. MMPs play an important role in wound healing, apoptosis, bone elongation, embryo development, angiogenesis, cancer metastases, and tissue remodeling within many disease states.
Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion.
Product Information
FormatAffinity Purified
Control
  • Rat lung lysate.
PresentationPurified in 0.1M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% NaN3.
Quality LevelMQ100
Applications
ApplicationDetect MT1-MMP using this Anti-MT1-MMP Antibody, hinge region validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Biological Information
ImmunogenKLH conjugated synthetic peptide selected from the hinge region of human MT1-MMP.
EpitopeHinge region
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThe antibody recognizes human and rat MT1-MMP. It does not cross react with MMP-1, MMP-2, MMP-8, MMP-9, and MMP-13.
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman and rat. Predicted to cross react with mouse, (95% sequence homology) and monkey chimpanzee, canine and bovine (100% sequence homology). Reactivity with other species has not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EC 3.4.24.80
  • MMP-14
  • MMP-X1
  • MT1-MMP
  • MT1MMP
  • MTMMP1
Purification MethodPeptide affinity purified
UniProt Number
UniProt SummaryFUNCTION: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
CATALYTIC ACTIVITY: Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.
COFACTOR: Binds 1 zinc ion per subunit By similarity.
Calcium By similarity.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Potential. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase By similarity.
SEQUENCE SIMILARITIES: Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Molecular Weight~ 65 kDa
Product Usage Statements
Quality AssuranceEvaluated on a representative lot by Western blot on rat lung lysate using Anti-MT1-MMP.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C for up to 1 year from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
AB6004 04053252620157