Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB2226 Anti-MDGA1 Antibody

Anti-MDGA1 Antibody detects level of MDGA1 & has been published & validated for use in Western Blotting.

Anti-MDGA1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: MDGA1, MAMDC3 UniProt Number: Q8NFP4
AB2226
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, B, HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB2226
DescriptionAnti-MDGA1 Antibody
Alternate Names
  • MAM domain-containing glycosylphosphatidylinositol anchor protein 1
  • GPI and MAM protein
  • GPIM
  • Glycosylphosphatidylinositol-MAM
  • MAM domain-containing protein 3
Background InformationStudies report that MDGA1 is a layer-specific marker and an area-specific marker, being expressed in layers 2/3 throughout the neocortex, but within the primary somatosensory area (S1), MDGA1 is also uniquely expressed in layers 4 and 6a. Comparisons with other markers, including cadherins, serotonin, cytochrome oxidase, RORß, and COUP-TF1, reveal unique features of patterned expression of MDGA1 within cortex and S1 barrels. Furthermore, during early stages of development, MDGA1 is expressed by Reelin- and Tbr1-positive Cajal–Retzius neurons that originate from multiple sources outside of neocortex and emigrate into it. At even earlier stages, MDGA1 is expressed by the earliest diencephalic and mesencephalic neurons, which appear to migrate from a MDGA1-positive domain of progenitors in the diencephalon and form a "preplate." These findings show that MDGA1 is a unique marker for studies of cortical lamination and area patterning and together with recent reports suggest that MDGA1 has critical functions in forebrain/midbrain development.
References
Product Information
FormatAffinity Purified
Control
  • Untreated and PNGase F treated mouse P7 brain tissue lysate.
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MDGA1 Antibody detects level of MDGA1 & has been published & validated for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the MAM domain of MDGA1.
EpitopeMAM domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the MAM domain of MDGA1.
Species Reactivity
  • Mouse
  • Rat
  • Bovine
  • Human
Species Reactivity NoteDemonstrated to react with Mouse and Rat. Predicted to react with Bovine and Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MDGA1
  • MAMDC3
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Required for radial migration of cortical neurons in the superficial layer of the neocortex.

SUBUNIT STRUCTURE: Interacts heterophilically through its MAM domain with proteins in axon-rich regions and through its Ig-like domains with proteins in differentiating muscle.

SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor › GPI-anchor. Note: Associated with lipid rafts.

TISSUE SPECIFICITY: Has been found in brain, heart, skeletal muscle and kidney. Found to be overexpressed in tumor tissues.
Molecular Weight~130kDa observed. UniProt describes 2 isoforms produced by alternative splicing at ~106 kDa (Isoform 1), ~107 kDa (Isoform 2). An uncharacterized band may be observed at ~48 kDa in some tissue lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in untreated and PNGase F treated mouse P7 brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MDGA1 in 5 µg of untreated and PNGase F treated mouse P7 brain tissue lysate. This antibody recognizes glycosolated (>160 kDa) (lane 1) and deglycosolated MDGA1 (lane 2).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB2226 04053252414015