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MAB1922 Anti-Laminin α2 Antibody, clone 5H2

MAB1922
100 µL  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Mk, RbELISA, CULT, IF, IHC, IP, WBMAscitesMonoclonal Antibody
Description
Catalogue NumberMAB1922
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Laminin α2 Antibody, clone 5H2
Alternate Names
  • Laminin M chain
  • Merosin heavy chain
  • laminin M
  • laminin alpha 2 subunit
  • laminin, alpha 2
Background InformationElements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14 - 19 with multiple developmental abnormalities. The kidney phenotypes include avascular glomeruli, impaired branching morphogenesis, and renal agenesis.
Product Information
FormatAscites
Control
  • Vascular breast carcinomas, Cultured embryonic retinal neurons and RGCs
PresentationUnpurified ascites in buffer containing no preservatives.
Quality LevelMQ100
Applications
ApplicationDetect Laminin α2 using this Anti-Laminin α2 Antibody, clone 5H2 validated for use in ELISA, CULT, IF, IH, IP & WB.
Key Applications
  • ELISA
  • Cell Culture
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesELISA:
50% maximal binding to human merosin at 1:50,000 dilution from a previous lot.

Immunohistochemistry:
1:5,000 dilution from a previous lot was used for staining of 8 µm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.

Immunofluorescence:
A previous lot of this antibody was used in immunofluorescent.

Affinity chromatography:
A previous lot of this antibody was used in IAP.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified human merosin
Clone5H2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the 80 kDa fragment of the M-chain of human merosin.
IsotypeIgG1
Species Reactivity
  • Human
  • Monkey
  • Rabbit
Species Reactivity NoteCross reacts with monkey and rabbit merosin.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryLaminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.
Gene Symbol
  • LAMA2
  • LAMM
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P24043 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
SIZE: 3110 amino acids; 342771 Da
SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-2 is a subunit of laminin-2 (merosin) and laminin-4 (S- merosin).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
TISSUE SPECIFICITY: Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
DOMAIN: SwissProt: P24043 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI, IV and G are globular.
DISEASE: SwissProt: P24043 # Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
SIMILARITY: Contains 17 laminin EGF-like domains. & Contains 5 laminin G-like domains. & Contains 2 laminin IV type A domains. & Contains 1 laminin N-terminal domain.
Molecular Weight80 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Human Placenta lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MAB1922 08436037123603