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MABF3183-25UG Anti-IL-1β Antibody, clone 11G7.3.7

Anti-IL-1β, clone 11G7.3.7, Cat. No. MABF3183, is a mouse monoclonal antibody that detects Interleukin-1β (IL-1β) and is tested for use in Immunohistochemistry (Paraffin) and in Western Blotting.

MABF3183-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3183-25UG
DescriptionAnti-IL-1β Antibody, clone 11G7.3.7
Alternate Names
  • Interleukin-1 beta
  • Catabolin
Background InformationInterleukin-1 beta (UniProt: P01584; also known as IL-1 beta, Catabolin) is encoded by the IL1B (also known as IL1F2) gene (Gene ID: 3553) in human. IL-1β is one of the best characterized potent pro-inflammatory cytokines that is critical for host-defense responses to infection and injury. It is produced and secreted by a variety of cell types; however, it is mainly expressed in activated monocytes/macrophages. IL-1β is produced as pro-IL-1β in response to molecular motifs carried by pathogens known as pathogen associated molecular patterns (PAMPs). Its synthesis occurs in two steps and each step is controlled by different stimuli. In the initial step, inflammatory signals, such as lipopolysaccharides, stimulate the synthesis and promote the accumulation of pro-IL-1β in cytoplasm. Following this step pro-IL-1β is cleaved by the caspase-1, the activation of which occurs via recruitment to inflammasome. This step removes the propeptide (1-116) segment. Following caspase-1-dependent processing of pro-IL-1β, mature IL-1β (aa 117-269) is rapidly secreted from cells. IL-1β is known to induce prostaglandin synthesis, neutrophil influx and activation, T-cell and B-cell activation and antibody production. It also promotes Th17 differentiation and synergizes with IL-12 to induce interferon g from T-helper cells. (Ref.: Lopez-Castejon, G., and Brough, D. (2011). Cytokine Growth Factor Rev. 22(4); 189-195; Tominaga, K., et al. (2000). Int. Immunol. 12(2); 151-160).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-IL-1β, clone 11G7.3.7, Cat. No. MABF3183, is a mouse monoclonal antibody that detects Interleukin-1β (IL-1β) and is tested for use in Immunohistochemistry (Paraffin) and in Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-1β in human lung tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length mature recombinant human Interleukin-1β (without the propeptide).
EpitopeUnknown
Clone11G7.3.7
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 11G7.3.7 is a mouse monoclonal antibody that detects human Interleukin-1β (IL-1β)
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL1B
Purification MethodProtein G purified
UniProt Number
Molecular Weight~17 kDa observed; 30.75 kDa calculated for the whole molecule. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant human Interleukin-1β (IL-1β).

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected human recombinant Interleukin-1β (IL-1β).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3183-25UG 04065267613918