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AB5312 Anti-Growth Associated Protein 43 Antibody

AB5312
100 µL  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, Mk, R, FeICC, IHC, IH(P), IP, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberAB5312
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Growth Associated Protein 43 Antibody
Alternate Names
  • GAP-43
  • Neuromodulin
  • B-50
Background InformationGAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
Product Information
FormatSerum
Control
  • Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons
PresentationSerum. Liquid with 0.02% sodium azide added as a preservative.
Quality LevelMQ100
Applications
ApplicationThis Anti-Growth Associated Protein 43 Antibody is validated for use in IC, IH, IH(P), IP, WB for the detection of Growth Associated Protein 43.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunocytochemistry: 1:100-1:400 for mouse primary neurons, NTera2 neurons.

Immuohistochemistry: 1:2,000 on coronal sections of adult visual cortex (McIntosh and Parkinson, 1990). AB5312 has been used to stain GAP-43 in 4% paraformaldehyde-fixed paraffin-embedded tissue sections of mouse olfactory bulb (Legrier, 2001).

Western blot: 1:500 - 1:1,000. Detects a single band at approximately 50 kDa on western blots of protein extracts from adult visual cortex (McIntosh and Parkinson, 1990).

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified GAP-43 from feline brain (McIntosh and Parkinson, 1990).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityAB5312 specifically recognizes GAP-43 regardless of the protein's phosphorylation state. This antiserum has been refered to in the literature as CG-1.
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Monkey
  • Rat
  • Feline
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system.
Gene Symbol
  • GAP43
  • PP46
  • Neuromodulin
  • B-50
  • neuromodulin
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons.
SIZE: 238 amino acids; 24803 Da
SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.
Molecular Weight23.6 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
AB5312 04053252270499