Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

371729 Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb

Anti-Giα-3-Subunit, C-Terminal (345-354), rabbit polyclonal, recognizes Giα-3 subunit. Does not cross-react with Gsα & Goα subunits. Validated for use in WB & IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
371729
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
MaRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
371729-50UL Glass bottle 50 ul
Description
OverviewRecognizes Giα-3 subunit. Does not cross-react with Gsα and Goα, but partially cross-reacts with Giα1-, G1α2-subunits.
Catalogue Number371729
Brand Family Calbiochem®
References
ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem. 262, 14,241.
Product Information
FormLiquid
FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:1000)
Immunoprecipitation (see comments)
Application CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH
HostRabbit
IsotypeIgG
Species Reactivity
  • Mammals
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
371729-50UL 04055977213300

Documentation

Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb Certificates of Analysis

TitleLot Number
371729

References

Reference overview
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem. 262, 14,241.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-August-2007 RFH
ApplicationImmunoblotting (1:1000)
Immunoprecipitation (see comments)
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the Giα-3 subunit protein.
HostRabbit
Immunogena synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH
IsotypeIgG
Speciesmammalian
FormLiquid
FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
PreservativeNone
CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem. 262, 14,241.