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05-758-I-25UG Anti-FE65 Antibody, clone 3H6

Anti-FE65, clone 3H6, Cat. No. 05-758-I, is a highly specific mouse monoclonal antibody that targets FE65 and has been tested for use in Western Blotting.

Anti-FE65 Antibody, clone 3H6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Apbb1, FE65 UniProt Number: Q9QXJ1
05-758-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-758-I-25UG
DescriptionAnti-FE65 Antibody, clone 3H6
Alternate Names
  • Amyloid-beta A4 precursor protein-binding family B member 1
  • Protein FE65
Background InformationAmyloid-beta A4 precursor protein-binding family B member 1 (UniProt: Q9QXJ1; also known as Protein FE65) is encoded by the Apbb1 (also known as FE65) gene (Gene ID: 11785) in murine species. FF65 is an adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. It is selectively expressed in the brain with high expression in regions affected in Alzheimer s disease. FE65 is phosphorylated at Threonine 579 by GSK-3 beta, which potentiates gamma and beta secretase-mediated APP processing and beta-amyloid liberation. This phosphorylation is also reported enhance FE65/APP complex formation. FE65 also plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. FE65 may act by specifically recognizing and binding histone H2AX phosphorylated on Tyrosine 142 (H2AXY142ph) at double-strand breaks, recruiting other pro-apoptosis factors such as MAPK8/JNK1. FE65 contains an N-terminal WW domain (aa 253-285) and two C-terminal phosphotyrosine interaction domains (aa 370-509, PID1 and aa 542-699, PID2). (Ref.: Lee, YS et al. (2017). Sci. Rep. 7(1):12456).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Applications
ApplicationAnti-FE65, clone 3H6, Cat. No. 05-758-I, is a highly specific mouse monoclonal antibody that targets FE65 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected FE65 in Tissue extracts of wild type (WT), Fe65 KO, Fe65L1 KO, and Fe65/Fe65L1 DKO mouse brains. (Courtesy of Univ. Prof. Dr. (PhD) Stefan Kins, Technical University of Kaiserslautern, Kaiserslautern, Germany).
Biological Information
ImmunogenGST-tgged recombinant fragment corresponding to 40 amino acids from the N-terminal half of murine Protein FE65.
Epitopecytoplasmic domain
Clone3H6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3H6 specifically detects Protein FE65 in murine and human cells.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Apbb1
  • FE65
Purification MethodProtein G purified
UniProt Number
Molecular Weight~97 kDa observed; 77.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotype Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-758-I-25UG 04054839468988