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MABC1128-25UG Anti-FAM83B Antibody, clone 7D11

Anti-FAM83B, clone 7D1, Cat. No. MABC1128, is a mouse monoclonal antibody that detects FAM83B and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-FAM83B Antibody, clone 7D11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: FAM83B, Q5T0W9 UniProt Number: Q5T0W9
MABC1128-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1128-25UG
DescriptionAnti-FAM83B Antibody, clone 7D11
Alternate Names
  • Protein FAM83B
  • Family with Sequence Similarity 83
  • member B
Background InformationProtein FAM83B (UniProt: Q5T0W9; also known as Family with Sequence Similarity 83, member B) is encoded by the FAM83B (also known as C6orf143) gene (Gene ID: 222584) in human. FAM83B is a member of an 8-member protein family (FAM83) that shares the highly conserved DUF1669. It is a proto-oncogene that functions in the epidermal growth factor (EGF) receptor signaling pathway. May activate both the EGFR itself and downstream RAS/MAPK and PI3K/AKT/TOR signaling cascades. It interacts with RAF1 leading to displacement of 14-3-3 proteins from RAF1, which then activates RAF1 within the RAS/MAPK signaling cascade. Higher levels of FAM83B are shown to increase PI3K/AKT signaling and cell proliferation. In EGFR-dependent breast cancer cells that express higher levels of FAM83B expression, ablation of FAM83B is shown to diminish p110 alpha and AKT membrane localization, which result in reduced AKT phosphorylation and cell proliferation. (Ref.: Cipriano, R., et al (2013). Oncotarget 4(5); 728-738; Cipriano, R., et al (2014). Oncogene 33(25); 3298-3306).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-FAM83B, clone 7D1, Cat. No. MABC1128, is a mouse monoclonal antibody that detects FAM83B and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected FAM83B in Western Blotting applications (Cipriano, R., et. al. (2014). Oncogene. 33(25):3298-306; Cipriano, R., et. al. (2012). J Clin Incest. 122(9):3197-210).

Immunocytochemistry Analysis: A representative lot detected FAM83B in Immunocytochemistry applications (Cipriano, R., et. al. (2012). J Clin Incest. 122(9):3197-210).

Immunoprecipitation Analysis: A representative lot detected FAM83B in Immunoprecipitation applications (Cipriano, R., et. al. (2014). Oncogene. 33(25):3298-306).
Biological Information
ImmunogenHis-tagged full length human recombinant Protein FAM83B.
Clone7D11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7D11 specifically detects human FAM83B protein.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FAM83B
  • Q5T0W9
Purification MethodProtein G purified
UniProt Number
Molecular Weight~115 kDa observed; 114.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293T cells transfected with SN3-hFAM83B-HA.

Western Blotting Analysis: 1 µg/mL of this antibody detected FAM83B in HEK293T cells transfected with SN3-hFAM83B-HA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1128-25UG 04054839510090