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MABT1507-100UL Anti-Desmoglein 2 Antibody, clone 6D8

Anti-Desmoglein 2, clone 6D8, Cat. No. MABT1507, is a mouse monoclonal antibody that detects Desmoglein-2 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition assays, and Western Blotting,

Anti-Desmoglein 2 Antibody, clone 6D8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: DSG2, CDHF5 UniProt Number: Q14126
MABT1507-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, IP, Inhibition, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT1507-100UL
DescriptionAnti-Desmoglein 2 Antibody, clone 6D8
Alternate Names
  • Cadherin family member 5
  • HDGC
Background InformationDesmoglein-2 (UniProt: Q14126; also known as Cadherin family member 5, HDGC) is encoded by the DSG2 (also known as CDHF5) gene (Gene ID: 1829) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-2 is a single-pass type I membrane protein that is found in all tissues and in carcinomas. It serves as a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are cleaved off in the mature form. It contains an extracellular domain (aa 50-609), a transmembrane domain (aa 610-634), and a cytoplasmic domain (aa 635-1118). Mutations in DSG2 gene have been linked to familial type of arrhythmogenic right ventricular dysplalsia that is characterized y infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
ApplicationAnti-Desmoglein 2, clone 6D8, Cat. No. MABT1507, is a mouse monoclonal antibody that detects Desmoglein-2 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition assays, and Western Blotting,
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunoprecipitation
  • Inhibition
  • Western Blotting
Application NotesFlow Cytometry Analysis: A representative lot detected Desmoglein 2 in Flow Cytometry applications (Tan, L.Y., et. al. (2016). Oncotarget. 7(29):46492-46508).

Western Blotting Analysis: A 1:125 dilution from a repesentative lot detected Desmoglein 2 in HaCaT cell lysates.

Flow Cytometry Analysis: 1 µg from a representative lot detected Desmoglein2 in SK-MEL-28.

Western Blotting Analysis: A representative lot detected Desmoglein 2 in Western Blotting applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44).

Immunocytochemistry Analysis: A representative lot detected Desmoglein 2 in Immunocytochemistry applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44; Tan, L.Y., et. al. (2016). Oncotarget. 7(29):46492-46508).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Desmoglein 2 in Immunoprecipitation applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44).

Inhibition Analysis: A representative lotinhibited the attachment of adenovirus (Ad) serotypes Ad3.(Wang, H., et. al. (2011). Nat Med. 17(1):96-104).
Biological Information
ImmunogenSupernatant from cell membranes extract from A-431 cells.
Clone6D8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6D8 specifically detects Desmoglein-2 in human cells.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DSG2
  • CDHF5
Purification MethodProtein G purified
UniProt Number
Molecular Weight~165 kDa observed; 122.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cell line.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Desmoglein 2 in A431 cell line.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1507-100UL 04054839531958