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MABE1126 Anti-DNA G-quadruplex (G4) Antibody, clone 1H6

This Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex .

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors UniProt Number: N/A
MABE1126
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AICC, IH(P), ELISA, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1126
DescriptionAnti-DNA G-quadruplex (G4) Antibody, clone 1H6
Alternate Names
  • DNA G-quadruplex (G4)
Background InformationSingle-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4). Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an appealing topic in molecular biomedical research.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex .
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • ELISA
  • Flow Cytometry
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DNA G-quadruplex (G4) in human pancreas and mouse kidney tissue.

Immunocytochemistry Analysis: A representative lot detected discrete DNA G-quadruplex (G4) foci on the compact chromosome in the metaphase HeLa and murine embryonic stem cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

Immunocytochemistry Analysis: A representative lot detected greatly diminished nuclear DNA G-quadruplex (G4) immunoreactivity upon DNase treatment of paraformaldehyde-fixed and Triton X-100-permeablized HeLa cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

Immunocytochemistry Analysis: A representative lot detected G-quadruplex (G4) stabilizing agent telomestatin/TMS treatment-induced nuclear DNA G4 immunoreactivity in chicken DT40 cells lacking the G4-unwinding helicase FANCJ, while the drug enhancing effect was not seen in human FANCJ-expressing DT40 cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

Immunocytochemistry Analysis: A representative lot detected a time-dependent increase of nuclear DNA G-quadruplex (G4) foci in U20S cells following the treatment of the DNA G4-stabilizing agent telomestatin/TMS (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

ELISA Analysis: Target selectivity of a representative lot was determined by competitive ELISA. Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

Flow Cytometry Analysis: A representative lot detected enhanced DNA G-quadruplex (G4) immunoreactivity in HeLa cells upon treatment with the DNA G4-stabilizing agent TMPyP4, Cat. No. 613560 (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

Immunohistochemistry Analysis: A representative lot detected nuclear DNA G-quadruplex (G4) immunoreactivity in various paraffin-embedded human tissue sections, including skin, pancreas, testis, placenta, brain, appendix, colon, and kidney (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Biological Information
ImmunogenKLH-conjugated G-quadruplex DNA derived from oligonucleotides with telomeric repeats.
Clone1H6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity, while exhibiting significantly lower affinity toward tetramolecular RNA structure or a triplex DNA structure, and little to no affinity toward either non-G4 ssDNA or ddDNA. However, clone 1H6 does not seem to bind the [AGGG(TTAGGG)3] unimolecular G4 structure, indicating a broad selectivity toward many, but not all, DNA G4 structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
IsotypeIgG2bκ
Species Reactivity
  • All
Species Reactivity NoteAll Species. Target DNA structure is not species-specific.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: 2.0 µg/mL of this antibody detected DNA G-quadruplex (G4) in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1126 04055977296464

Documentation

Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 SDS

Title

Safety Data Sheet (SDS) 

Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 Certificates of Analysis

TitleLot Number
Anti-DNA G-quadruplex (G4), -2728267 2728267
Anti-DNA G-quadruplex (G4), -2778846 2778846
Anti-DNA G-quadruplex (G4), -2789412 2789412
Anti-DNA G-quadruplex (G4), -2816634 2816634
Anti-DNA G-quadruplex (G4), -Q2613645 Q2613645
Anti-DNA G-quadruplex (G4), clone 1H6 - 2990486 2990486
Anti-DNA G-quadruplex (G4), clone 1H6 - 3226258 3226258
Anti-DNA G-quadruplex (G4), clone 1H6 - 3389940 3389940
Anti-DNA G-quadruplex (G4), clone 1H6 - 3450598 3450598
Anti-DNA G-quadruplex (G4), clone 1H6 - 3595117 3595117
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References

Reference overviewPub Med ID
Detection of G-quadruplex DNA in mammalian cells.
Henderson, A; Wu, Y; Huang, YC; Chavez, EA; Platt, J; Johnson, FB; Brosh, RM; Sen, D; Lansdorp, PM
Nucleic acids research  42  860-9  2014

Show Abstract 		24163102 24163102

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