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AB356480-25UL Anti-Cas9 (Rabbit Polyclonal)

Anti-Cas9 (Rabbit Polyclonal), Cat. No. AB356480, is a highly specific rabbit polyclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunofluorescence and Western Blotting.

Anti-Cas9 (Rabbit Polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Cas9 UniProt Number: J7RUA5
AB356480-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
BactIF, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberAB356480-25UL
DescriptionAnti-Cas9 (Rabbit Polyclonal)
Alternate Names
  • SaCas9
  • CRISPR-associated endonuclease Cas9
Background InformationCRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9, Cas9) is encoded by the Cas9 gene in Streptococcus aureus. Cas9 belongs to subtype II-A subfamily with one HNH Cas9-type domain. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Streptococcus pyogenes is not closely related in amino acid sequence in S. aureus, hence this antibody does not recognize that protein.
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-Cas9 (Rabbit Polyclonal), Cat. No. AB356480, is a highly specific rabbit polyclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells with transfected  with  GFP-Cas9-SA (Courtesy of Dr. Gerry Shaw). Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells overexpressing fusion protein GFP-Cas9 (Courtesy of Dr. Gerry Shaw).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 251 amino acids from the C-terminal end of CRISPR-associated endonuclease Cas9 from Staphylococcus aureus.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Cas9 from Staphylococcus aureus. It targets an epitope within 251 amino acids from the C-terminal end. It does not react with Cas9 from Streptococcus pyogenes.
IsotypeIgG
Species Reactivity
  • Bacteria
Species Reactivity NoteStaphylococcus aureus
Antibody TypePolyclonal Antibody
Gene Symbol
  • Cas9
Purification MethodUnpurified
UniProt Number
Molecular Weight~55 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells overexpressing fusion protein GFP-Cas9.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Cas9 (Rabbit Polyclonal) in 40 µg of H EK293 cells overexpressing fusion protein GFP-Cas9 from Staphylococcus aureus.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB356480-25UL 04054839518652