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MABS2260-100UL Anti-CLEC18A Antibody, clone 3A9E6

Anti-CLEC18A, clone 3A9E6, Cat. No. MABS2260, is a mouse monoclonal antibody that detects C-type lectin domain family 18 member A (CLEC18A) and is tested in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

MABS2260-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2260-100UL
DescriptionAnti-CLEC18A Antibody, clone 3A9E6
Alternate Names
  • C-type lectin domain family 18 member A
  • Mannose receptor-like protein 2
Background InformationC-type lectin domain family 18 member A (UniProt: A5D8T8; also known as Mannose receptor-like protein 2, CLEC18A) is encoded by the (also known as MRLP2) gene (Gene ID: 348174) in human. CLEC18A is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form (aa 27-446). Its expression is observed in all cells and in peripheral blood cells. It is mainly localized in the endoplasmic reticulum, Golgi apparatus, and endosome. Although it is expressed in normal hepatocytes, it is absent in hepatocellular carcinoma. It binds polysaccharides in a Ca2+-independent manner and shows preferential binding to fucoidal, b-glucans and galactans. It also serves as a co-receptor for TLR3 and contributes to the differential immune responses to poly(I:C) and H5N1 influenza A virus infection. Its levels are shown to be up-regulated upon differentiation of monocytes into macrophages and dendritic cells. Significantly higher levels of CLEC18A are observed subjects with hepatitis C infection and this increased level is associated with increased cryoglobulin levels, which may be related to the occurrence of mixed cryobulinemia (MC) syndrome. (Ref.: Huang, YL., et al. (2021). Nat. Commun. 4;229; Liao, TL., et al. (2018). Sci. Rep. 8; Article 17287; Huang, YL., et al. (2015). J. Biol. Chem. 290(35); 21252-21263).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-CLEC18A, clone 3A9E6, Cat. No. MABS2260, is a mouse monoclonal antibody that detects C-type lectin domain family 18 member A (CLEC18A) and is tested in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63).

ELISA Analysis: A representative lot detected CLEC18A in ELISA applications (Liao T.L. et al. (2018). Sci Rep. 8(1):17287).

Western Blotting Analysis: A representative lot detected CLEC18A in Western Blotting applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Huang, Y.L, et al. (2021). Commun Biol. 4(1):229).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CLEC18A in human kidney tissue sections.

Flow Cytometry Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Liao T.L. et al. (2018). Sci Rep. 8(1):17287).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified recombinant human C-type lectin domain family 18 member A (CLEC18A) Fc fusion protein.
EpitopeUnknown
Clone3A9E6
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3A9E6 is a mouse monoclonal antibody that detects human C-type lectin domain family 18 member A (CLEC18A).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CLEC18A
Purification MethodProtein G purified
UniProt Number
Molecular Weight~54 kDa observed; 49.6 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U937 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CLEC18A in U937 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2260-100UL 04065266876383