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MABF2006-100UG Anti-CHIKV CP Antibody, clone 5.5G9

Anti-CHIKV CP, clone 5.5G9, Cat. No. MABF2006, is a mouse monoclonal antibody that detects CHIKV Capsid protein (CP) and has been tested for use in ELISA, Immunohistochemistry and Western Blotting.

MABF2006-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF2006-100UG
DescriptionAnti-CHIKV CP Antibody, clone 5.5G9
Alternate Names
  • Capsid protein CHIKV
  • Chikungunya virus capsid protein
Background InformationChikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. This monoclonal antibody (clone 5.5G9) can bind strongly to full length as well as a series of N-truncations (N1-N4). It can recognize capsid protein even in the absence of the first 140 residues. (Ref.: Goh, LYH et al., (2015). Viruses. 7(6); 2943-2964; Goh, LYH et al., (2015). J. Gen. Virol. 96(3); 507-512).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
Applications
ApplicationAnti-CHIKV CP, clone 5.5G9, Cat. No. MABF2006, is a mouse monoclonal antibody that detects CHIKV Capsid protein (CP) and has been tested for use in ELISA, Immunohistochemistry and Western Blotting.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected CHIKV CP in Immunohistochemistry applications (Goh, L.Y.H., et. al. (2015). J Gen Virol. 96(Pt 3):507-12).

Western Blotting Analysis: A representative lot detected CHIKV CP in Western Blotting applications (Goh, L.Y.H., et. al. (2015). Viruses. 7(6):2943-64).

ELISA Analysis: A representative lot detected CHIKV CP in ELISA applications (Goh, L.Y.H., et. al. (2015). J. Gen. Virol. 96(Pt 3):507-12).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected CHIKV CP in C6/36 mosquito cell lysates either mock-infected (negative) or CHIKV-infected (positive). (Courtsey of Jody Peters, Ph.D.., University of Queensland, Australia).
Biological Information
ImmunogenPurified inactivated capsid protein from CHIKV Mauritius strain (CHIKVMAU), challenged with live virus (CHIKVTHAI), followed by a final boost with inactivated antigen (CHIKVTHAI).
Clone5.5G9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5.5G9 specifically detects capsid protein in Chikungunya virus. It detects N-truncations (N1-4) and the full-length capsid protein.
IsotypeIgG2a
Species Reactivity
  • Virus
Species Reactivity NoteChikungunya virus.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight~30 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed to be mouse IgG2a by isotype testing.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2006-100UG 04054839646751