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MABF2182-100UL Anti-CD46 Antibody, clone TRA-2-10

Anti-CD46, clone TRA-2-10, Cat. No. MABF2182, is a mouse monoclonal antibody that detects Membrane cofactor protein (CD46) and has been for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Inhibition assay.

MABF2182-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2182-100UL
DescriptionAnti-CD46 Antibody, clone TRA-2-10
Alternate Names
  • Membrane cofactor protein
  • TLX
  • Trophoblast leukocyte common antigen
Background InformationMembrane cofactor protein (UniProt: P15529; also known as TLX, Trophoblast leukocyte common antigen, CD46) is encoded by the CD46 (also known as MCP; MIC10) gene (Gene ID: 4179) in human. CD46 is a single-pass type I membrane protein that is expressed by all cells except erythrocytes. It acts as a cofactor for complement factor I, a serine protease that protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. CD46 is synthesized with a signal peptide (aa 1-34), which is cleaved off in the mature form. The mature form contains an extracellular domain (aa 35-343), a transmembrane domain (aa 344-366), and a cytoplasmic domain (aa 367-392). It also contains four Sushi domains where Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles Virus H protein, domains 2 and 3 are essential for Herpes virus 6 binding, and domains 3 and 4 are required for interaction with Streptococcus Pyrogens M protein and are the most important for interaction with C3b and C4b. CD46 can be glycosylated on Asn 83, 114, and 273 and N-glycosylation on Asn 114 and 273 are required for its cytoprotective function. N-glycosylation on Asn 114 is also required for Measles virus binding and N-glycosylation on Asn-273 is required for Neisseria binding. CD46 can also be extensively O-glycosylated in the Ser/Thr-rich domain, which is required for Neisseria binding, but not for Measles virus binding. Mutations in CD46 gene have been linked to hemolytic uremic syndrome atypical 2 that has been characterized by microangiopathic hemolytic anemia, thrombocytopenia, and renal failure.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
Applications
ApplicationAnti-CD46, clone TRA-2-10, Cat. No. MABF2182, is a mouse monoclonal antibody that detects Membrane cofactor protein (CD46) and has been for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Inhibition assay.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry
  • Inhibition
Application NotesInhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).

Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).

Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Biological Information
Immunogen2102Ep human embryonal carcinoma cells.
CloneTRA-2-10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone TRA-2-10 specifically detects CD46 in human cells.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD46
  • MCP
  • MIC10
Purification MethodProtein G purified
UniProt Number
Molecular Weight43.75 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD46 in one million HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2182-100UL 04054839577130

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