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MABF418 Anti-CD4 Antibody (human), PerCP-Cy5.5, clone OKT4

This mouse monoclonal antibody Anti-CD4 (human), PerCP-Cy5.5, clone OKT4 validated for use in western blotting, IP, ICC, Immunofluorescence & Flow Cytometry for the detection of CD4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD4 UniProt Number: P01730
MABF418
100 tests  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, IHC, IP, WBMPerCP-Cy5.5Monoclonal Antibody
Description
Catalogue NumberMABF418
DescriptionAnti-CD4 Antibody (human), PerCP-Cy5.5, clone OKT4
Alternate Names
  • T-cell surface glycoprotein CD4
  • T-cell surface antigen T4/Leu-3
  • CD antigen CD4
Background InformationThe OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The OKT4 antibody is widely used as a phenotypic marker for CD4 expression. It is cross-reactive with CD4 in several non-human species, including Chimpanzee, Cynomolgus and Rhesus. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065)
Product Information
FormatPerCP-Cy5.5
PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal antibody Anti-CD4 (human), PerCP-Cy5.5, clone OKT4 validated for use in western blotting, IP, ICC, Immunofluorescence & Flow Cytometry for the detection of CD4.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneOKT4
Concentration5 µL (0.25 µg)/test
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CD4
Purification MethodSize Exclusion Chromatography
UniProt Number
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD4 in one million human peripheral blood lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 tests
Global Trade Item Number
Catalogue Number GTIN
MABF418 04053252960093