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217660 Anti-CD18 Mouse mAb (IB4)

Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.

Anti-CD18 Mouse mAb (IB4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-β2 Integrin

217660
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
217660-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
Catalogue Number217660
Brand Family Calbiochem®
SynonymsAnti-β2 Integrin
References
ReferencesBerman, M.E., et al. 1996. J. Immunol. 156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Negative controlNalm-6 cells
Positive controlHPB-MLT cells
Preservative0.5 mg/ml gentamicin sulfate
Quality LevelMQ100
Applications
Key Applications Cell Inhibition Assays
Flow Cytometry
Frozen Sections
Immunoprecipitation
Application NotesFlow Cytometry (5-10 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Cell Inhibition Assays (see comments)
Application CommentsBlocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman peripheral blood monocytes
ImmunogenHuman
CloneIB4
HostMouse
Purity≥95% by SDS-PAGE
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
217660-100UG 04055977202946

Documentation

Anti-CD18 Mouse mAb (IB4) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD18 Mouse mAb (IB4) Certificates of Analysis

TitleLot Number
217660

References

Reference overview
Berman, M.E., et al. 1996. J. Immunol. 156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-July-2009 JSW
SynonymsAnti-β2 Integrin
ApplicationFlow Cytometry (5-10 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Cell Inhibition Assays (see comments)
DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
BackgroundHuman CD18 (β2 integrin) is expressed on leukocytes as part of a heterodimeric complex with either CD11a, b or c (integrins αL, αM, or αX). CD18/CD11 heterodimers function in cell adhesion in immune and inflammatory responses.
HostMouse
Immunogen speciesHuman
Immunogenhuman peripheral blood monocytes
CloneIB4
IsotypeIgG2a
Specieshuman
Positive controlHPB-MLT cells
Negative controlNalm-6 cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Preservative0.5 mg/ml gentamicin sulfate
CommentsBlocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesBerman, M.E., et al. 1996. J. Immunol. 156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.