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MABF2003-25UG Anti-CD11a Antibody, clone BU17

Anti-CD11a, clone BU17, Cat. No. MABF2003, is a mouse monoclonal antibody that detects CD11a and has been tested for use in Flow Cytometry.

Anti-CD11a Antibody, clone BU17 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: ITGAL, CD11A UniProt Number: P20701
MABF2003-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2003-25UG
DescriptionAnti-CD11a Antibody, clone BU17
Alternate Names
  • Integrin alpha-L
  • CD11 antigen-like family member A
  • Leukocyte adhesion glycoprotein LFA-1 alpha chain
  • LFA-1A
  • Leukocyte function-associated molecule 1 alpha chain
Background InformationIntegrin alpha-L (UniProt: P20701; also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain, CD11a) is encoded by the ITGAL (also known as CD11A) gene (Gene ID: 3683) in human. Integrins are part of a family of heterodimeric transmembrane glycoproteins that recognize a variety of ligands and control numerous physiologic functions in cells. CD11a is a single-pass type I membrane protein that noncovalently associates with beta2 integrin to forms a heterodimer and serve as a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils, and eosinophils. It is also involved in generation of common lymphoid progenitor cells in bone marrow. In resting T-cells, about 40% of CD11a is constitutively phosphorylated and phosphorylation causes conformational changes needed for ligand binding and is considered to be essential for activation by some physiological agents. CD11a is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off in the mature form. It has a large extracellular domain (aa 26-1090), a transmembrane domain (aa 1091-1111), and a cytoplasmic domain 1112-1170). Three isoforms of CD11a have been described that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-CD11a, clone BU17, Cat. No. MABF2003, is a mouse monoclonal antibody that detects CD11a and has been tested for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A representative lot detected CD11a in Flow Cytometry applications (Baker, M.P., et. al. (1998). Blood. 92(8):2830-43).
Biological Information
ImmunogenWaldenstrom macroglobulinemia lymphoplasmacytic cells.
Epitopeextracellular domain
CloneBU17
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone BU17 detects CD11a in human cells. It targets an epitope with in the extracellular domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITGAL
  • CD11A
Purification MethodProtein G purified
UniProt Number
Molecular Weight128.77 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in one million human peripheral blood mononuclear cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD11a in one million human peripheral blood mononuclear cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2003-25UG 04054839573064