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MABN801 Anti-BRAP Antibody, clone 3C18

Anti-BRAP, clone 3C18 Antibody, Cat. No. MABN801, is a highly specific mouse monoclonal antibody that targets BRAP and has been tested in Immunocytochemistry and Western Blotting.

Anti-BRAP Antibody, clone 3C18 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Brap UniProt Number: Q99MP8
MABN801
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Mk, H, MICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN801
DescriptionAnti-BRAP Antibody, clone 3C18
Alternate Names
  • BRCA1-associated protein
  • BRAP2
  • IMP
  • Impedes mitogenic signal propagation
Background InformationBRCA1-associated protein (UniProt Q99MP8; also known as BRAP2, IMP, Impedes mitogenic signal propagation) is encoded by the Brap gene (Gene ID 72399) in murine species. BRAP is a Ras effector that controls the sensitivity of MAPK signaling by negatively regulating the MAPK scaffold protein KSR1 (kinase suppressor of Ras 1), allowing adaptations and homeostasis in a complex tissue environment. In the developing CNS, mitogenic/morphogenic molecules from location-specific signaling centers form counteracting gradients and simultaneously guide orchestrated cell proliferation, neuronal differentiation, and tissue morphogenesis. Consistent with its role as a MAPK signaling threshold modulator, BRAP2 is shown to interact with both Nde1 and Lis1 to form a complex that provides proper buffer strength to set up the signaling threshold according to the local concentration of mitogens and morphogens. The Nde1-Lis1-BRAP complex helps prevent excessive activation of MAPK under high local mitogenic activity in the midline and promote the self-renewal of progenitors that are programmed to generate a large cohort of neurons, but are localized distant from the midline.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-BRAP, clone 3C18 Antibody, Cat. No. MABN801, is a highly specific mouse monoclonal antibody that targets BRAP and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: 5 µg/mL from a representative lot detected EGFP-tagged mouse BRAP in transfected COS-7 cells (Courtesy of Yuanyi Feng, Ph.D., Northwestern University, USA).

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected exogenously overexpressed mouse BRAP in 25-50 µg of lysate from transfected HEK293T cells (Courtesy of Yuanyi Feng, Ph.D., Northwestern University, USA).

Immunocytochemistry Analysis: A representative lot detected BRAP immunoreactivity co-localized with that of LIS1 beneath the plasma membrane by fluorescent immunocytochemistry staining of mouse embryonic fibroblasts (MEFs), 293FT and COS1 cells (Lanctot, A.A., et al. (2013). Dev. Cell. 25(3):241-255).

Western Blotting Analysis: A representative lot detected both the endogenous human BRAP in untransfected HEK293T as well as the exogenously expressed myc-tagged murine BRAP in transfected HEK293T cells (Lanctot, A.A., et al. (2013). Dev. Cell. 25(3):241-255).

Note: DO NOT HEAT SAMPLES PRIOR TO GEL LOADING. BRAP protein tends to form insoluble aggregates upon heating.
Biological Information
ImmunogenRecombinant murine BRAP.
Clone3C18
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3C18 specifically detected the target band in wild-type, but not Brap-knockout MEF lysate. Clone 3C18 epitope region is absent in human spliced isoform 2 and mouse spliced isoform 3 (UniProt Q7Z569-2 and Q99MP8-3).
IsotypeIgG1κ
Species Reactivity
  • Monkey
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse, Monkey. Predicted to react with Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Brap
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~67 kDa observed. 67.31 kDa (human isoform 1), 66.99/63.95 kDa (mouse isoform 1/2; isoform alpha/beta), 65.58 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvalulated by Western Blotting in mouse BRAP-expressing HEK293T cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected exogenously expressed mouse BRAP in 20 µg of lysate from transfected HEK293T cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN801 04054839055522