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MABD411 Anti-BMP4 Antibody, clone PA354-16.1.1

This Anti-BMP4 antibody is validated for use in WB, FC, IH for the detection of BMP4.

Anti-BMP4 Antibody, clone PA354-16.1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: BMP4, BMP2B, DVR4 UniProt Number: P12644
MABD411
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABD411
DescriptionAnti-BMP4 Antibody, clone PA354-16.1.1
Alternate Names
  • Bone morphogenetic protein 4
  • BMP-4
  • Bone morphogenetic protein 2B
  • BMP-2B
Background InformationBone morphogenetic protein 4 (BMP4), also known as Bone morphogenetic protein 4 (BMP-4), Bone morphogenetic protein 2B (BMP-2B), and encoded by the gene name BMP4/ BMP2B/ DVR4, is morphogenetic protein that induces cartilage and bone formation. Similar to other bone morphogenetic proteins, BMP4 has been shown to play an important role in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-BMP4 antibody is validated for use in WB, FC, IH for the detection of BMP4.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BMP4 in human pancreas tissue.
Flow Cytometry Analysis: 1:200 dilution of this antibody from a representative lot detected BMP4 in Caco-2 cells (Data courtesy of Juan Ignacio Imbaud, Protein Alternatives).
Biological Information
ImmunogenMBP-tagged recombinant protein corresponding to human BMP4.
ClonePA354-16.1.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BMP4
  • BMP2B
  • DVR4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight47 kDa calculated. The calculated molecular weight is 47 kDa, however BMP4 has been shown as a ~80 and ~90 kDa band in western blots with the use of a MBP tag.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human recombinant protein BMP4.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.01 µg human recombinant protein BMP4.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABD411 04055977116953