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MABS1304 Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8

MABS1304
100 μg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICC, ELISA, DBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1304
DescriptionAnti-ATP Synthase subunit β Antibody, clone 11/21-7-A8
Alternate Names
  • ATP synthase subunit beta, mitochondrial
  • ATP Synthase subunit β
  • beta-F1-ATPase
Background InformationATP synthase subunit beta, mitochondrial (EC 3.6.3.14; UniProt P06576; also known as ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide, beta-F1-ATPase, Epididymis secretory protein Li 271, Mitochondrial ATP synthase beta subunit, Mitochondrial ATP synthetase beta subunit) is encoded by the ATP5B (also known as ATPMB, ATPSB, HEL-S-271) gene (Gene ID 506) in human. Mitochondrial ATP synthase produces ATP from ADP in the presence of a proton gradient generated by electron transport complexes. This ATPase contains two structural domains, F1-containing extramembrane catalytic core, and F0-containing the membrane proton channel that are linked via a central stalk and a peripheral stalk. Subunits alpha and beta form the catalytic code in F1. Tumor development requires the selection of cancer cells with a repressed biogenesis and functional activity of mitochondria. Both beta-F1-ATPase/GAPDH and beta-F1-ATPase/Hsp60 ratios are found to be significantly lower in tumors than the corresponding normal tissues. Studies conducted in human colon cancer cell line HCT116 show the involvement of AMPK (AMP-activated protein kinase) and GCN2 (general control non-derepressible 2; eIF2α kinase) in the onset of colon cancer progression by repressing of beta-F1-ATPase synthesis and promoting the abnormal bioenergetics of mitochondria.
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8 is validated for use in Western Blotting, Immunocytochemistry, ELISA and Dot Blot for the detection of ATP Synthase subunit β.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • ELISA
  • Dot Blot
Application NotesImmunocytochemistry Analysis: A representative lot immunostained mitochondrial tubular network in human breast cancer Hs578T cells (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
ELISA Analysis: A representative lot detected His-tagged full-length human ATP Synthase subunit β (beta-F1-ATPase) recombinant protein by direct ELISA (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Dot Blot Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) by Dot blot using His-tagged full-length human beta-F1-ATPase recombinant protein or HepG2 lysate (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) in human hepatoma HepG2, murine hepatoma Hepa 1-6, and normal rat liver epithelial C9 (Clone 9) cells.
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) expression in various cancer patients tissues (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) downregulation in HCT116 human colon cancer cells in response to AMPK pathway activation upon oligomycin or AICAR treatment (Martinez-Reyes, J., et al. (2012). Biochem J. 444(2):249-259).
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to human ATP Synthase subunit β.
Clone11/21-7-A8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATP5B
  • ATPMB
  • ATPSB
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~56 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATP Synthase subunit β in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Global Trade Item Number
Catalogue Number GTIN
MABS1304 04055977277388