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07-363 Anti-AMPK α2 Antibody

07-363
200 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWBRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-363
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-AMPK α2 Antibody
Alternate Names
  • AMP-Activated Protein Kinase
  • AMPK
Product Information
FormatPurified
Control
  • Rat liver preparation
PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationDetect AMPK α2 using this Anti-AMPK α2 Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogenpeptide corresponding to amino acids 353-367 (C-DDSAMHIPPGLKPHP) of human AMP-Activated Protein Kinase alpha2 (AMPKalpha2) catalytic subunit
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes AMP-Activated Protein Kinase alpha2 (AMPKα2).
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Gene Symbol
  • PRKAA2
  • AMPK
  • AMPK2
  • PRKAA
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P54646 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
COFACTOR: Magnesium.
SIZE: 552 amino acids; 62320 Da
SUBUNIT: Heterotrimer of a catalytic subunit, a beta and a gamma non-catalytic subunits.
SIMILARITY: SwissProt: P54646 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
Molecular Weight~63 kDa
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot in a rat liver preparation
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size200 µg
Global Trade Item Number
Catalogue Number GTIN
07-363 04053252468735