Chromolith® Prep |
Up-Scaling
With Chromolith® Prep monolithic columns, analytical separations can be easily transferred to preparative chromatography by linear transfer of methods. The objective of any preparative separation strategy is high sample throughput per unit of time. Therefore, columns are often run under concentration and/or volume overload conditions. However, the maximum load on the column is dependent on the complexity of the separation and the nature of the sample.
Calculating the Flow Rate or Injection Volume
Whether working in a linear or non-linear mode, the flow rate or injection volume is calculated according to the equation below.
Transfer from Analytical to Preparative Column
The table depicts guide values of typical flow rates and loading capacity for the transfer from an analytical to a preparative column.
| Column | Column dimension [length/diameter] | Typical flow rate | Loading capacity | Loading volume |
|---|---|---|---|---|
| Analytical column | 100-4.6 mm | 2 mL/min | 5 mg | 5-50 μL |
| Preparative column | 100-25 mm | 60 mL/min | 150-370 mg | 100-1500 μL |
Chromolith® Prep Product Range
The ready-to-use columns are cladded with a polymeric material (PEEK), and can be connected directly to any HPLC system.
Product |
Ordering Number |
|---|---|
| Chromolith® Prep Si | 125251 |
| Chromolith® Prep RP-18-e | 125252 |
| Chromolith® Prep Guard Cartridge Si | 125260 |
| Chromolith® Prep Guard Cartridge RP-18e | 125261 |
| Chromolith® Prep Sealing Set (2 O-rings) | 125254 |
| Chromolith® Prep Tool Set (1 mounting tool filter, 1 mounting tool, 1 hook wrench) | 125255 |
| Chromolith® Prep Endcap Set (1 inlet cap complete, 1 outlet cap) | 125256 |
| Chromolith® Prep Frit Set (10 frits) | 125257 |
| Chromolith® Prep 25 mm Column Couple | 125259 |
Related Resources
